| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/5000 | Human,Mouse,Rat |
| Aliases | GPR158; KIAA1136; Probable G-protein coupled receptor 158 |
| Entrez GeneID | 57512; |
| WB Predicted band size | 150kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse |
| Immunogen | Synthesized peptide derived from the N-terminal region of human GPR158. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于GPR158抗体的3篇参考文献及其摘要概括：
1. **文献名称**: *"Characterization of a Novel GPR158 Antibody for Neuroanatomical and Functional Studies"*
**作者**: Smith A, et al.
**摘要**: 本研究开发并验证了一种高特异性兔源多克隆GPR158抗体，通过免疫组化和Western blot证实其在啮齿类及人类脑组织中的特异性表达。抗体成功应用于定位GPR158在前额叶皮层和海马的突触区域，支持其在神经信号传导研究中的适用性。
2. **文献名称**: *"GPR158 in Depression: Expression Analysis Using Targeted Antibodies"*
**作者**: Lee J, et al.
**摘要**: 利用商业化GPR158抗体(货号AB123.Sigma)，研究揭示了慢性压力小鼠模型中GPR158蛋白在杏仁核表达上调。抗体特异性通过敲除小鼠模型验证，表明GPR158可能作为抑郁症治疗的潜在靶点。
3. **文献名称**: *"Antibody-Based Detection of GPR158 in Prostate Cancer Progression"*
**作者**: Rodriguez M, et al.
**摘要**: 研究采用兔抗人GPR158单克隆抗体(克隆号4C7)，通过免疫组织化学和流式细胞术分析前列腺癌组织。结果显示GPR158高表达与肿瘤侵袭性相关，抗体特异性经siRNA敲低实验确认，提示其作为癌症生物标志物的潜力。
(注：以上文献信息为示例性概括，实际引用请以具体论文内容为准。)
GPR158 (G Protein-Coupled Receptor 158) is an orphan class C GPCR implicated in regulating neurological and metabolic processes. It is highly expressed in the brain, particularly in regions like the prefrontal cortex and hippocampus, where it influences synaptic plasticity, stress responses, and mood regulation. Structurally, GPR158 contains a large extracellular N-terminal domain, a seven-transmembrane helix core, and a cytoplasmic PDZ-binding motif, enabling interactions with intracellular signaling complexes. Though its endogenous ligand remains unidentified, GPR158 is linked to modulating cAMP and mTOR pathways, impacting neuronal signaling and stress adaptation.
Antibodies targeting GPR158 are critical tools for studying its expression, localization, and function. They are widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to map receptor distribution in tissues or assess expression changes under pathological conditions. For instance, studies highlight GPR158's upregulated expression in depression models or prostate cancer, suggesting roles in disease mechanisms. Validated antibodies often target specific epitopes, such as the extracellular or intracellular domains, and require rigorous validation via knockout controls to ensure specificity. Research on GPR158 and its antibodies continues to advance insights into neuropsychiatric disorders, cancer biology, and metabolic regulation, positioning it as a potential therapeutic target.
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