| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | FANCD2; FACD; Fanconi anemia group D2 protein; Protein FACD2 |
| Entrez GeneID | 2177; |
| WB Predicted band size | 166kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from human FANCD2 around the non-phosphorylation site of S222. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于FANCD2抗体的3篇代表性文献，按文献名称、作者和摘要内容概括列出：
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1. **文献名称**：*Functional Interaction of Monoubiquitinated FANCD2 and BRCA2/FANCD1 in Vertebrate Cells*
**作者**：Nijman SM, et al.
**摘要**：该研究通过使用FANCD2特异性抗体，揭示了FANCD2单泛素化修饰在DNA损伤修复中的关键作用，并证明其与BRCA2/FANCD1的相互作用对维持基因组稳定性至关重要。
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2. **文献名称**：*A Conserved Ubiquitin Ligase of the Fanconi Anemia Core Complex*
**作者**：Garcia-Higuera I, et al.
**摘要**：作者利用FANCD2抗体在细胞中验证了范可尼贫血核心复合物(FA core complex)对FANCD2单泛素化的调控，阐明了其在DNA损伤应答中的分子机制。
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3. **文献名称**：*FANCD2 Localizes to Mitotic Structures and Is Required for Chromosomal Stability*
**作者**：Chan KL, et al.
**摘要**：通过免疫荧光和Western blot实验(使用FANCD2抗体)，研究发现FANCD2在有丝分裂期间定位于染色体和纺锤体，并证明其缺陷会导致染色体分离异常和基因组不稳定性。
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4. **文献名称**：*The Fanconi Anemia Pathway Promotes Replication-Dependent DNA Interstrand Cross-Link Repair*
**作者**：Knipscheer P, et al.
**摘要**：该文献通过FANCD2抗体的应用，证实了范可尼贫血通路在复制依赖性DNA链间交联修复中的作用，并揭示了FANCD2单泛素化与修复效率的直接关联。
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以上文献均聚焦于FANCD2蛋白的功能及抗体在其研究中的关键应用，涵盖DNA修复、泛素化调控及细胞周期机制等领域。如需具体期刊信息或年份，可进一步补充检索。
The FANCD2 antibody is a critical tool in studying the Fanconi anemia (FA) pathway, a DNA repair mechanism essential for maintaining genomic stability. FANCD2. a key protein in this pathway, plays a pivotal role in repairing DNA interstrand cross-links (ICLs) and coordinating homologous recombination repair. Upon DNA damage, FANCD2 is monoubiquitinated (forming FANCD2-L) and relocates to chromatin, where it facilitates repair processes. Antibodies targeting FANCD2 are widely used to detect its expression, monoubiquitination status, and subcellular localization, providing insights into FA pathway activation.
These antibodies are vital in research on Fanconi anemia, a rare genetic disorder characterized by bone marrow failure, cancer predisposition, and developmental defects. They help diagnose FA subtypes by assessing FANCD2 protein levels or modifications in patient cells. Additionally, FANCD2 antibodies are employed in cancer biology studies, as FA pathway defects are linked to chemotherapy resistance and tumorigenesis. They are also used to explore interactions between FANCD2 and other DNA repair proteins, such as BRCA1/2.
Common applications include Western blotting, immunofluorescence, and flow cytometry. Specificity for either the ubiquitinated (FANCD2-L) or non-ubiquitinated (FANCD2-S) form allows researchers to distinguish pathway activation states. Validated FANCD2 antibodies are essential for ensuring reproducibility in studies of DNA damage response mechanisms and their clinical implications.
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