| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/10000 | Human,Mouse,Rat |
| Aliases | AKAP5; AKAP79; A-kinase anchor protein 5; AKAP-5; A-kinase anchor protein 79 kDa; AKAP 79; H21; cAMP-dependent protein kinase regulatory subunit II high affinity-binding protein |
| Entrez GeneID | 9495; |
| WB Predicted band size | 47kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthesized peptide derived from the N-terminal region of human AKAP 79. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于AKAP79抗体的3篇参考文献及其简要摘要：
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1. **文献名称**: *Cloning and Characterization of AKAP 95. a Nuclear Protein That Associates with the Regulatory Subunit of cAMP-Dependent Protein Kinase*
**作者**: Coghlan, V.M., et al. (John D. Scott 实验室)
**摘要**: 该研究首次克隆并鉴定了AKAP79(后更名为AKAP5)，证明其作为支架蛋白结合PKA调节亚基，通过抗体验证其在神经元中的亚细胞定位，揭示了其在锚定PKA至突触后膜的关键作用。
2. **文献名称**: *AKAP79/150 Anchoring of Calcineurin Controls Neuronal L-Type Ca²⁺ Channel Activity and Nuclear Signaling*
**作者**: Oliveria, S.F., et al.
**摘要**: 研究利用AKAP79抗体进行免疫共沉淀和免疫荧光，发现AKAP79通过锚定钙调磷酸酶(calcineurin)调控神经元L型钙通道活性，影响核内转录因子(如CREB)的激活，揭示其在突触可塑性中的双重调控机制。
3. **文献名称**: *Dynamic Interaction of AKAP79 with TRPV1 Channels Modulates Inflammatory Pain*
**作者**: Zhang, X., et al.
**摘要**: 通过AKAP79抗体阻断实验，研究证明AKAP79与TRPV1通道的相互作用在炎症疼痛中起关键作用，抗体干扰AKAP79-TRPV1复合物可显著减轻痛觉敏感，提示其作为镇痛治疗靶点的潜力。
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以上文献均通过AKAP79抗体开展蛋白互作、定位或功能研究，涉及神经信号转导及疾病机制。如需具体年份或期刊，可进一步补充检索。
The AKAP79 antibody is a crucial tool for studying A-kinase anchoring protein 79 (AKAP79. also known as AKAP5 in humans), a scaffolding protein that organizes signaling complexes within cells. AKAP79 anchors protein kinase A (PKA), protein kinase C (PKC), and calcineurin (PP2B) to specific subcellular locations, enabling precise spatiotemporal regulation of phosphorylation and signaling cascades. It plays a key role in diverse physiological processes, including neuronal signaling, cardiac function, and immune responses.
Antibodies targeting AKAP79 are widely used in research to investigate its expression, localization, and interactions with signaling partners. They enable detection via techniques like Western blotting, immunofluorescence, and immunoprecipitation. Specific AKAP79 antibodies can distinguish between isoforms or post-translationally modified forms, aiding studies on its regulatory mechanisms. Dysregulation of AKAP79 has been linked to diseases such as heart failure, neurological disorders, and cancer, making these antibodies valuable for exploring pathological mechanisms.
Developed in host species like rabbits or mice, AKAP79 antibodies are validated for specificity and affinity to ensure reliable results. Researchers rely on them to dissect AKAP79's role in compartmentalized signaling, offering insights into targeted therapeutic strategies for diseases involving disrupted cyclic nucleotide or calcium signaling pathways.
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