| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/300 | Human,Mouse,Rat |
| ICC | 1/200-1/1000 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 1/20000 | Human,Mouse,Rat |
| Aliases | ACTG2; ACTA3; ACTL3; ACTSG; Actin; gamma-enteric smooth muscle; Alpha-actin-3; Gamma-2-actin; Smooth muscle gamma-actin |
| Entrez GeneID | 72; |
| WB Predicted band size | 45kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Synthesized peptide derived from the N-terminal region of human Actin α3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,0.5%BSA and 50% glycerol. |
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以下是关于Actin α3抗体的示例参考文献(注：以下为构造示例，建议通过学术数据库获取真实文献)：
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1. **标题**: *"A monoclonal antibody specific for α3-actin in smooth muscle differentiation"*
**作者**: Müller et al.
**摘要**: 研究报道了一种特异性识别平滑肌α3-actin的单克隆抗体，验证了其在发育中小鼠模型中的表达模式，证实其在区分平滑肌细胞与其他肌动蛋白亚型中的特异性。
2. **标题**: *"Actin α3 as a biomarker in pulmonary arterial hypertension: Antibody-based detection and clinical relevance"*
**作者**: Chen et al.
**摘要**: 通过定制抗体分析肺高压患者组织样本，发现Actin α3表达水平与疾病严重程度相关，提示其作为潜在生物标志物的价值。
3. **标题**: *"Generation of a polyclonal antibody against human ACTA3 and its application in gastrointestinal stromal tumors"*
**作者**: Tanaka et al.
**摘要**: 开发了针对人源ACTA3的多克隆抗体，用于免疫组化检测胃肠道间质瘤中的表达，发现其与肿瘤迁移能力相关。
4. **标题**: *"Cross-reactivity analysis of commercial Actin α3 antibodies: Implications for cardiac fibrosis studies"*
**作者**: Johnson et al.
**摘要**: 评估了多种市售Actin α3抗体的交叉反应性，强调部分抗体在心脏纤维化模型中可能与其他肌动蛋白亚型发生非特异性结合，提出了优化实验方案的建议。
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建议通过PubMed、Google Scholar等平台，以关键词“Actin α3 antibody”“ACTA3 antibody”或结合研究领域(如“smooth muscle”“cancer”)检索最新文献。
The actin alpha 3 (ACTα3) antibody is a tool used to detect the α3 isoform of actin, a key component of the cytoskeleton. ACTα3. encoded by the *ACTA3* gene, belongs to the α-skeletal muscle actin family and is predominantly expressed in skeletal muscle tissues. It plays a critical role in muscle contraction by interacting with myosin filaments. Mutations in *ACTA3* are linked to congenital myopathies, such as nemaline myopathy and congenital fiber-type disproportion, making the antibody valuable for studying these disorders.
The ACTα3 antibody is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess protein expression, localization, and integrity in muscle samples. It helps researchers distinguish α3 from other actin isoforms (e.g., α1 and α2), aiding in tissue-specific studies. Validation often includes reactivity checks against recombinant ACTα3 and cross-reactivity assessments with other isoforms to ensure specificity.
In research, this antibody contributes to understanding muscle development, regeneration, and pathology. It is employed to investigate disease mechanisms, monitor therapeutic interventions, and analyze muscle differentiation in cell models. Commercial ACTα3 antibodies are typically raised in hosts like rabbits or mice, with clonality (monoclonal/polyclonal) varying by supplier. Proper controls, such as knockout tissue or isoform-specific peptides, are recommended to confirm signal accuracy. Overall, the ACTα3 antibody is a vital reagent for advancing studies in neuromuscular biology and translational medicine.
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