| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Cyan fluorescent protein; Enhanced Cyan fluorescent protein |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Formulation | Purified antibody in TBS with 0.05% sodium azide and 50% glycerol. |
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以下是关于ECFP(Enhanced Cyan Fluorescent Protein)Rabbit抗体的参考文献示例，内容基于模拟文献概括：
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1. **文献名称**：*Development and Validation of a Rabbit Polyclonal Antibody for Enhanced Cyan Fluorescent Protein Detection*
**作者**：Smith J, et al.
**摘要**：本研究报道了一种新型兔多克隆抗体的开发，该抗体特异性识别ECFP标签蛋白。通过免疫印迹(Western blot)和免疫荧光实验验证了其高亲和力和低交叉反应性，适用于活细胞成像和蛋白质定位研究。
2. **文献名称**：*Application of ECFP-Specific Rabbit Antibodies in Multiplexed Fluorescence Imaging*
**作者**：Lee H, et al.
**摘要**：文章探讨了ECFP兔抗体在多重荧光标记系统中的性能，证明其与红色和绿色荧光蛋白标记系统的兼容性，为多通道生物标记分析提供了可靠工具。
3. **文献名称**：*Comparative Analysis of ECFP Antibody Performance Across Species*
**作者**：Garcia R, et al.
**摘要**：比较了不同宿主来源(兔、小鼠)的ECFP抗体性能，结果显示兔源抗体在稳定性和信号强度上表现更优，尤其在组织切片染色中背景噪音更低。
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注：以上文献为示例性内容，实际引用时需检索真实数据库(如PubMed、Web of Science)获取准确信息。若ECFP指代其他靶标(如特定信号分子)，建议结合具体研究背景调整检索关键词。
The ECFP (Enhanced Cyan Fluorescent Protein) Rabbit antibody is a polyclonal antibody developed to specifically detect and bind to the ECFP, a engineered variant of green fluorescent protein (GFP) derived from *Aequorea victoria*. ECFP emits cyan fluorescence (λem ≈ 476 nm) and is widely used as a reporter protein in molecular and cellular biology for tracking gene expression, protein localization, and dynamic cellular processes.
Rabbit-derived antibodies against ECFP are generated by immunizing rabbits with purified ECFP or its immunogenic peptide sequences, followed by affinity purification to ensure specificity. These antibodies exhibit high affinity and minimal cross-reactivity with other fluorescent protein variants (e.g., EGFP, EYFP), making them ideal for applications requiring precise detection of ECFP-tagged proteins.
Common applications include Western blotting, immunofluorescence (IF), immunohistochemistry (IHC), and co-immunoprecipitation (Co-IP) in studies involving recombinant proteins, gene editing (e.g., CRISPR/Cas9 reporters), or live-cell imaging. The antibody’s robustness in diverse experimental conditions, coupled with compatibility with secondary antibodies conjugated to fluorophores or enzymes, enhances its utility in multiplex assays.
As fluorescent protein tags grow indispensable in biomedical research, ECFP Rabbit antibodies remain critical tools for validating fusion protein expression, studying protein interactions, and visualizing subcellular dynamics with high resolution. Their reliability and versatility support advancements in fields like neurobiology, cancer research, and developmental biology.
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