Mouse Monoclonal PARPMouse Antibody

PARP (Poly(ADP-ribose) polymerase) antibodies, particularly those derived from mice, are essential tools for studying the role of PARP proteins in DNA repair, genomic stability, and programmed cell death. PARP-1. the most well-characterized member of this enzyme family, detects single-strand DNA breaks and catalyzes poly-ADP-ribosylation to recruit repair proteins. Mouse monoclonal anti-PARP antibodies are widely used to detect PARP expression, cleavage, or activity in various experimental models, including cancer research and apoptosis studies. These antibodies often target specific epitopes, such as the 89 kDa cleavage fragment generated by caspases during apoptosis, serving as a marker for cell death. They are applied in techniques like Western blotting, immunohistochemistry, and immunofluorescence to assess PARP involvement in DNA damage responses, chemotherapy resistance, or the efficacy of PARP inhibitors in BRCA-mutant cancers. The development of PARP inhibitors (e.g., olaparib) for targeted cancer therapy has further increased the demand for reliable PARP detection tools. Mouse-derived antibodies offer high specificity and consistency, making them critical for mechanistic studies and therapeutic evaluation. Their use has significantly advanced understanding of PARP's dual roles in cell survival (DNA repair) and death (apoptosis), as well as its implications in neurodegenerative diseases and inflammation.