Rabbit Polyclonal RNF8(Ab-198) Antibody

The RNF8 (Ab-198) antibody is a research tool designed to detect and study the RNF8 protein, a key E3 ubiquitin ligase involved in DNA damage response (DDR) pathways. RNF8 contains a RING finger domain critical for its enzymatic activity and plays a pivotal role in the repair of DNA double-strand breaks (DSBs). It operates by ubiquitinating target proteins, such as H2A histones, facilitating the recruitment of downstream repair factors like BRCA1 and 53BP1 to damage sites. This antibody, often validated for applications like Western blotting, immunofluorescence, and immunoprecipitation, helps researchers investigate RNF8's expression, localization, and functional interactions in cellular contexts. Dysregulation of RNF8 has been linked to genomic instability, cancer progression, and resistance to therapies targeting DNA repair. Studies using RNF8 (Ab-198) have contributed to understanding its role in cell cycle checkpoints, chromatin remodeling, and crosstalk with other DDR proteins like ATM and RNF168. Its specificity for human, mouse, or rat RNF8 (depending on the product datasheet) makes it valuable in translational research exploring cancer biology, genotoxic stress responses, and potential therapeutic targets.