关键信息
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基因名
KCNMB3
- 应用
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别名
KCNMA1;KCNMA;SLO;Calcium-activated potassium channel subunit alpha-1
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种属
Human
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表达系统
E. coli
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标签
His tag N-Terminus
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纯度
Greater than 90% as determined by SDS-PAGE.
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蛋白编号
Q9NPA1
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表达区间
82-207aa
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氨基酸序列
MGSSHHHHHHSSGLVPRGSHMGSKPFMLSIQREESTCTAIHTDIMDDWLD CAFTCGVHCHGQGKYPCLQVFVNLSHPGQKALLHYNEEAVQINPKCFYTP KCHQDRNDLLNSALDIKEFFDHKNGTPFSCFYSPASQSEDVILIKKYDQ
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分子量
17 kDa
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内毒素
< 1.0 EU per μg protein as determined by the LAL method.
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性状
Freeze-dried powder
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缓冲液
PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
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复溶方法
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
- 个性化定制
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稳定性测试
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37℃ for 48h, and no obvious degradation and precipitation were observed. The loss rate isless than 8% within the expiration date under appropriate storage condition.
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保存条件 & 期限
Samples are stable for up to twelve months from date of receipt at -20℃ to -80℃. Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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运输条件
In general, recombinant proteins are supplied as lyophilized powder and shipped at ambient temperature. For bulk packages, the proteins are provided as frozen liquid and shipped with blue ice, unless otherwise requested by the customer.
质检流程
相关产品
背景信息
KCNMB3, also known as the beta subunit of the calcium-activated potassium channel (KCa) family, plays a crucial role in modulating the activity of KCa channels, particularly in smooth muscle and neuronal tissues. The understanding of KCNMB3 is significant due to its involvement in various physiological processes, including vasodilation, neurotransmission, and neuronal excitability. Abnormalities in KCNMB3 function have been linked to various pathological conditions, such as hypertension and neurodegenerative diseases. Recombinant KCNMB3 protein is often produced to study its structural properties, functional mechanisms, and interactions with other channel subunits and signaling molecules. Advances in protein expression systems and purification techniques have facilitated the generation of high-quality recombinant KCNMB3, allowing researchers to conduct detailed investigations into its biophysical characteristics and pharmacological profiles. Understanding the role of KCNMB3 is vital for developing potential therapeutic strategies targeting KCa channels, offering insights into the modulation of vascular tone and neuronal signaling, ultimately contributing to better management of cardiovascular and neurological disorders.
