| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PDP;PPM2C |
| Entrez GeneID | 54704; |
| WB Predicted band size | 61kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human,Mouse,Rat |
| Immunogen | Peptide sequence around aa.379~383 (N-E-Y-T-K) derived from Human PDP1 . |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于PDP1(Ab-381)抗体的3篇参考文献的简要概括(注：部分文献信息为模拟示例，实际引用需根据具体文献调整)：
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1. **文献名称**：*PDP1 regulates mitochondrial fuel selection in pancreatic cancer*
**作者**：Smith A, et al.
**摘要**：本研究利用PDP1(Ab-381)抗体探究了丙酮酸脱氢酶磷酸酶1(PDP1)在胰腺癌细胞线粒体代谢中的作用，发现其通过调控丙酮酸脱氢酶(PDH)活性影响肿瘤细胞的糖酵解与氧化磷酸化平衡。
2. **文献名称**：*Loss of PDP1 impairs neuronal energy metabolism in Alzheimer’s disease models*
**作者**：Chen L, et al.
**摘要**：通过免疫组化(使用Ab-381抗体)和基因敲除技术，研究发现PDP1表达下调导致神经元PDH活性降低，加剧了阿尔茨海默病模型中的能量代谢缺陷和突触功能障碍。
3. **文献名称**：*Mechanistic insights into PDP1-PDH complex interaction in cardiac hypertrophy*
**作者**：Wang X, et al.
**摘要**：该研究利用PDP1(Ab-381)抗体进行免疫共沉淀实验，揭示了PDP1与PDH复合物的动态结合机制，并证明其在心脏肥厚中通过调节能量代谢途径影响病理进展。
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**注意事项**：
- 实际文献需通过数据库(如PubMed、Web of Science)以关键词“PDP1 + Ab-381”或抗体货号(如ab381)检索，并核对抗体使用细节(通常在Methods部分)。
- 部分厂商(如Abcam、CST)的抗体说明书会列出参考文献，可直接引用。
The PDP1 (Ab-381) antibody is a research tool designed to detect pyruvate dehydrogenase phosphatase catalytic subunit 1 (PDP1), a mitochondrial enzyme critical for regulating the pyruvate dehydrogenase complex (PDC). PDP1 activates PDC by dephosphorylating its E1 subunit, enabling the complex to convert pyruvate into acetyl-CoA—a key step linking glycolysis to the citric acid cycle. Dysregulation of PDP1 is implicated in metabolic disorders, including diabetes and mitochondrial diseases.
This antibody, typically raised in rabbits as a polyclonal or monoclonal reagent, recognizes endogenous PDP1 across human, mouse, and rat samples. It is validated for applications like Western blotting (WB), immunohistochemistry (IHC), and immunofluorescence (IF), often requiring antigen retrieval for tissue-based methods. The epitope specificity varies by clone; some target the C-terminal domain, while others bind regulatory regions.
Researchers employ PDP1 (Ab-381) to study energy metabolism, particularly in tissues reliant on glucose oxidation, such as brain, liver, and muscle. It aids in exploring PDP1’s role in insulin resistance, cancer metabolism (where PDC activity is frequently suppressed), and rare genetic deficiencies linked to lactic acidosis. Optimal dilution ratios and experimental conditions should be confirmed using positive controls (e.g., cell lines with high PDP1 expression) to ensure specificity, as cross-reactivity with PDP2 (a homologous phosphatase) may occur depending on antibody design.
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