Rabbit Polyclonal p7S6Kinase(Ab-411) Antibody

The p70S6 Kinase (p70S6K), also known as ribosomal protein S6 kinase beta-1 (S6K1), is a serine/threonine kinase that plays a critical role in regulating cell growth, proliferation, and survival by integrating signals from the mTOR (mechanistic target of rapamycin) pathway. It exists in two isoforms (p70 and p85) and is activated via phosphorylation at multiple sites, including Thr389 (a key regulatory site) and Ser411. among others. The p70S6 Kinase (Ab-411) antibody specifically detects the phosphorylated form of p70S6K at Ser411. a residue associated with its activation or functional modulation in response to growth factors, nutrients, or cellular stress. This antibody is widely used in research to study mTORC1 signaling dynamics, particularly in contexts such as cancer, metabolism, and autophagy. Its applications include Western blotting, immunoprecipitation, and immunofluorescence to assess kinase activity, pathway activation, or responses to mTOR inhibitors (e.g., rapamycin). Researchers often employ this antibody to investigate how dysregulation of p70S6K contributes to diseases like diabetes, obesity, and malignancies, where aberrant mTOR signaling is implicated. Proper validation using knockout controls or phosphorylation-blocking assays is recommended to ensure specificity.