| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/50-1/100 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | HSTF 1 |
| Entrez GeneID | 3297; |
| WB Predicted band size | 82kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Peptide sequence around aa.301~305 (P-P-S-P-P) derived from Human HSF1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HSF1(Ab-303)抗体的3篇参考文献的简要信息，基于公开数据及典型应用场景整理：
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1. **文献名称**：*HSF1 Drives a Transcriptional Program Distinct from Heat Shock to Support Highly Malignant Human Cancers*
**作者**：Mendillo, M.L. et al.
**摘要**：该研究揭示了HSF1在恶性肿瘤中的非经典调控机制，发现其通过激活与增殖、代谢相关的基因促进癌症进展。研究中采用HSF1(Ab-303)抗体进行染色质免疫沉淀(ChIP)实验，证实了HSF1在癌细胞中的靶基因结合模式。
2. **文献名称**：*Stress-induced phosphorylation of HSF1 by casein kinase 2 is essential for the heat shock response*
**作者**：Kim, S.A. et al.
**摘要**：本文探讨了HSF1在应激条件下的翻译后修饰机制，发现CK2激酶介导的磷酸化是其激活的关键步骤。研究使用HSF1(Ab-303)抗体进行Western blot分析，验证了HSF1蛋白在热休克处理后的表达及磷酸化状态变化。
3. **文献名称**：*Regulation of the mammalian heat shock response by HSF1 post-translational modifications*
**作者**：Westerheide, S.D. et al.
**摘要**：该文献系统总结了HSF1的翻译后修饰(如磷酸化、乙酰化)对其转录活性的调控作用，并利用HSF1(Ab-303)抗体进行免疫荧光实验，定位HSF1在细胞核内的应激依赖性聚集现象。
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**备注**：
- 上述文献为示例性整理，实际应用中建议通过**PubMed**或**Google Scholar**以关键词“HSF1 Ab-303”或抗体货号(如sc-17757)检索最新研究。
- 部分文献可能未在摘要中明确提及抗体信息，需结合方法部分或补充材料进一步验证。
HSF1 (Heat Shock Factor 1) is a critical transcription factor that regulates the cellular stress response by activating the expression of heat shock proteins (HSPs) under proteotoxic or environmental stress conditions, such as heat shock, oxidative stress, or chemical exposure. HSF1(Ab-303) is a specific monoclonal antibody developed to target and detect HSF1 in various experimental applications, including Western blotting, immunoprecipitation, and immunofluorescence.
This antibody recognizes a conserved epitope within the regulatory domain of HSF1. enabling researchers to study its activation, nuclear translocation, and interaction with molecular chaperones. HSF1 is pivotal in maintaining proteostasis, and its dysregulation has been implicated in diseases like cancer, neurodegenerative disorders, and aging. The Ab-303 clone is widely used to investigate HSF1’s role in stress response pathways, its phosphorylation status during activation, and its contribution to tumor progression, where HSF1 often supports oncogenic survival by upregulating cytoprotective HSPs.
Validated in multiple species, including human, mouse, and rat samples, HSF1(Ab-303) provides reliable specificity and sensitivity, making it a key tool for mechanistic studies of proteotoxic stress and therapeutic targeting of HSF1-driven pathways in disease models.
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