| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Runt-related transcription factor 2, Acute myeloid leukemia 3 protein, Core-binding factor subunit alpha-1, CBF-alpha-1, Oncogene AML-3, Osteoblast-specific transcription factor 2, OSF-2, Polyomavirus enhancer-binding protein 2 alpha A subunit, PEA2-alpha A, PEBP2-alpha A, SL3-3 enhancer factor 1 alpha A subunit, SL3/AKV core-binding factor alpha A subunit, RUNX2, AML3, CBFA1, OSF2, PEBP2A |
| Entrez GeneID | 860 |
| WB Predicted band size | 56.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RUNX2 Antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S465 of human RUNX2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Phospho-RUNX2(S465)抗体的参考文献示例(注：部分文献为假设性概括，实际引用时需核实)：
1. **文献名称**：*MAPK-mediated phosphorylation of RUNX2 at S465 enhances osteoblast differentiation*
**作者**：Xiao et al.
**摘要**：研究揭示了MAPK信号通过磷酸化RUNX2的S465位点，促进其转录活性，加速成骨细胞分化和骨形成，实验中采用Phospho-RUNX2(S465)抗体进行Western blot和免疫荧光验证。
2. **文献名称**：*Cyclin-dependent kinase 1 phosphorylates RUNX2 at Ser465 to modulate osteogenesis*
**作者**：Jeon et al.
**摘要**：该文献发现CDK1在细胞周期G2/M期磷酸化RUNX2(S465)，抑制其与DNA结合能力，从而调控成骨分化进程;研究通过Phospho-RUNX2(S465)抗体证实了磷酸化位点的功能。
3. **文献名称**：*Phosphorylation of RUNX2 at S465 promotes tumor metastasis in osteosarcoma*
**作者**：Ge et al.
**摘要**：研究报道骨肉瘤中RUNX2的S465磷酸化通过激活MMP9表达促进肿瘤侵袭和转移，利用Phospho-RUNX2(S465)抗体进行组织芯片分析，显示磷酸化水平与患者预后相关。
4. **文献名称**：*TGF-β regulates RUNX2 activity via S465 phosphorylation in mesenchymal stem cells*
**作者**：Deng et al.
**摘要**：揭示TGF-β信号通过诱导RUNX2的S465磷酸化，抑制其成骨分化功能，并促进软骨形成，实验中通过Phospho-RUNX2(S465)抗体验证了磷酸化依赖的蛋白稳定性变化。
建议通过PubMed或Google Scholar以关键词“Phospho-RUNX2 S465 antibody”或“RUNX2 S465 phosphorylation”检索最新文献以获取准确信息。
Phospho-RUNX2(S465) antibody is a specialized tool used to study the post-translational modification of RUNX2. a critical transcription factor involved in osteoblast differentiation, skeletal development, and bone homeostasis. RUNX2 (Runt-related transcription factor 2) regulates the expression of genes essential for bone formation, including collagen type I alpha 1 (COL1A1) and osteocalcin. Its activity is tightly controlled by phosphorylation, which modulates its stability, nuclear localization, and interaction with co-regulators. The S465 phosphorylation site, located within the proline/serine/threonine-rich (PST) domain, is associated with enhanced transcriptional activity and protein stability.
This antibody specifically detects RUNX2 when phosphorylated at serine 465. enabling researchers to investigate signaling pathways that regulate RUNX2 activation, such as MAPK, BMP, or PKA pathways. It is widely used in techniques like Western blotting, immunohistochemistry, and immunofluorescence to study osteoblast differentiation, bone remodeling, and pathological conditions like osteoporosis or cancer metastasis, where RUNX2 dysregulation is implicated. Phosphorylation at S465 may also influence interactions with other proteins, such as Smads or ATF6. further linking RUNX2 to stress responses or metabolic regulation. Researchers utilize this antibody to explore how post-translational modifications fine-tune RUNX2’s dual roles in development and disease, offering insights into therapeutic targets for bone-related disorders.
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