Rabbit Polyclonal SPTBN1 Antibody

The SPTBN1 gene encodes βII-spectrin, a crucial component of the spectrin family that contributes to the maintenance of cell membrane integrity, cytoskeletal organization, and intracellular signaling. βII-spectrin is ubiquitously expressed and plays roles in cell adhesion, polarity, and vesicle trafficking by linking membrane proteins to the actin cytoskeleton. Antibodies targeting SPTBN1 are essential tools for studying its expression, localization, and function in both normal and pathological contexts. These antibodies are widely used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate βII-spectrin's involvement in diseases, including cancer, neurological disorders, and cardiovascular conditions. For instance, altered SPTBN1 expression has been linked to tumor progression, metastasis, and chemoresistance, while mutations are associated with neurodevelopmental defects. Researchers also utilize SPTBN1 antibodies to explore its interaction partners, such as ankyrins and actin, to dissect molecular pathways. Challenges include ensuring antibody specificity due to structural similarities among spectrin isoforms and post-translational modifications. Validations using knockout cell lines or siRNA-mediated knockdown are often recommended. Commercially available SPTBN1 antibodies are typically developed in hosts like rabbit or mouse, with monoclonal and polyclonal variants offering distinct advantages in sensitivity and applicability. Overall, these antibodies remain indispensable for advancing insights into cellular dynamics and disease mechanisms tied to βII-spectrin dysfunction.