Rabbit Polyclonal Phospho-FAS(Y291) Antibody

The Phospho-FAS (Y291) antibody detects FAS (CD95/APO-1) when phosphorylated at tyrosine 291. a post-translational modification implicated in regulating FAS-mediated signaling. FAS, a member of the tumor necrosis factor receptor (TNFR) superfamily, is a cell surface death receptor that triggers apoptosis upon binding to its ligand (FASL). This process involves receptor trimerization, recruitment of adaptor proteins like FADD, and activation of caspase cascades. Phosphorylation at Y291. located in the intracellular death domain of FAS, has been suggested to modulate its apoptotic activity. Studies indicate that tyrosine phosphorylation may influence FAS receptor clustering, internalization, or interaction with signaling partners, potentially acting as a regulatory switch to either enhance or suppress apoptosis depending on cellular context. Aberrant FAS signaling is linked to cancer, autoimmune disorders, and neurodegenerative diseases. The Phospho-FAS(Y291) antibody serves as a tool to investigate phosphorylation-dependent mechanisms in FAS-mediated cell death, particularly in studies exploring resistance to apoptosis in malignancies or dysregulated immune responses. It is commonly used in techniques like Western blotting, immunofluorescence, or flow cytometry to assess phosphorylation status under experimental conditions such as ligand stimulation, kinase inhibition, or stress-induced apoptosis. Validation typically includes testing in FAS-expressing cell lines with or without phosphatase treatment.