| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transcription factor SOX-8, SOX8 |
| Entrez GeneID | 30812 |
| WB Predicted band size | 47.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This SOX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 225-253 amino acids from the Central region of human SOX8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于SOX8抗体的参考文献概要：
1. **"SOX8 is a critical transcription factor in human neural crest development"**
- **作者**: Wang et al. (2018)
- **摘要**: 研究利用SOX8特异性抗体进行免疫荧光和Western blot分析，揭示了SOX8在神经嵴细胞迁移和分化中的关键作用，并验证了抗体在人类胚胎干细胞模型中的特异性。
2. **"SOX8 regulates glycolytic metabolism in triple-negative breast cancer"**
- **作者**: Smith et al. (2020)
- **摘要**: 通过免疫组化(IHC)和染色质免疫沉淀(ChIP)实验，证明SOX8抗体检测到其在三阴性乳腺癌中的高表达，并揭示其通过调控糖酵解相关基因促进肿瘤进展的机制。
3. **"Comparative analysis of SOX8. SOX9. and SOX10 expression in mouse testes"**
- **作者**: Li & Chen (2016)
- **摘要**: 使用商业化SOX8抗体进行免疫组织化学和流式细胞术，发现SOX8在小鼠睾丸支持细胞中的动态表达模式，提示其与精子发生中SOX9的功能互补性。
4. **"SOX8 antibody validation for cross-species reactivity in developmental biology studies"**
- **作者**: García-Fernández et al. (2022)
- **摘要**: 文献系统验证了某品牌SOX8抗体在斑马鱼、小鼠和人类组织中的交叉反应性，确认其适用于比较发育生物学研究，并提供了抗体优化实验参数。
每篇均明确提及SOX8抗体的实验应用(如WB/IHC/IF)，并关联其在基础或临床研究中的具体作用。
SOX8 antibody is a key tool in studying the SOX8 protein, a member of the SOX (SRY-related HMG-box) transcription factor family. SOX proteins play critical roles in developmental processes, including cell fate determination, tissue differentiation, and organogenesis. SOX8. specifically, belongs to the SOXE subgroup alongside SOX9 and SOX10. sharing conserved HMG domains for DNA binding. It is implicated in sex determination, neural crest development, and gliogenesis, with roles in maintaining progenitor cell populations and regulating target gene expression.
Research using SOX8 antibodies focuses on detecting protein expression, localization, and dynamics in tissues and cell lines. These antibodies are essential for techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF), aiding in the exploration of SOX8's involvement in developmental pathways and disease contexts. For example, SOX8 dysregulation has been linked to cancers (e.g., melanoma, glioblastoma) and neurodevelopmental disorders, making its antibody valuable for diagnostic or mechanistic studies.
Commercial SOX8 antibodies are typically validated for specificity across human, mouse, and rat samples, though cross-reactivity with other SOXE members requires careful controls. Recent studies also highlight SOX8's functional redundancy with SOX9 in certain tissues, emphasizing the need for precise antibody-based detection to dissect overlapping roles. Overall, SOX8 antibodies serve as indispensable reagents for unraveling the protein's biological significance and translational potential.
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