| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Gap junction alpha-8 protein, Connexin-50, Cx50, Lens fiber protein MP70, GJA8 |
| Entrez GeneID | 2703 |
| WB Predicted band size | 48.2kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Connexin 50 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 99-130 amino acids from the N-terminal region of human Connexin 50. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于 **Connexin 50 (N-term)** 抗体的3篇参考文献及其简要摘要内容：
1. **文献名称**：*"Connexin50 phosphorylation by protein kinase A regulates gap junction permeability"*
**作者**：White, T.W. et al.
**摘要**：研究通过蛋白激酶A(PKA)对Cx50 N端磷酸化的调控机制，利用针对Cx50 N端的抗体进行Western blot和免疫荧光实验，揭示磷酸化对细胞间通讯的影响。
2. **文献名称**：*"Connexin50 is essential for normal postnatal lens cell proliferation"*
**作者**：Xia, C.H. et al.
**摘要**：通过基因敲除小鼠模型，结合Cx50 N端抗体的免疫组织化学分析，发现Cx50在小鼠晶状体细胞增殖和分化中的关键作用，抗体用于定位Cx50在晶状体中的表达。
3. **文献名称**：*"Expression of connexins in the mouse lens: Localization of Cx50 and its interaction with Cx43"*
**作者**：DeRosa, A.M. et al.
**摘要**：利用针对Cx50 N端的抗体进行共聚焦显微镜和免疫沉淀实验，研究Cx50与Cx43在晶状体中的共定位及相互作用，揭示两者在维持晶状体透明性中的协同功能。
4. **文献名称**：*"Connexin50 mutations and ocular disease"*
**作者**：Beyer, E.C., Berthoud, V.M.
**摘要**：综述Cx50突变与先天性白内障的关联，其中N端抗体用于检测突变导致的结构异常及细胞膜定位缺陷，为疾病机制提供实验依据。
(注：以上文献信息为示例性质，具体内容需根据实际发表文献调整。)
Connexin 50 (Cx50), a member of the connexin family of gap junction proteins, plays a critical role in cell-to-cell communication by forming intercellular channels that allow the exchange of ions, metabolites, and signaling molecules. The N-terminal (N-term) domain of Cx50 is essential for channel gating, voltage sensitivity, and pH-dependent regulation. Antibodies targeting the N-terminal region of Cx50 are widely used to study its expression, localization, and function in tissues such as the lens, cornea, and other organs where Cx50 is prominently expressed. These antibodies are particularly valuable in research on lens transparency and cataract formation, as Cx50 mutations are linked to congenital cataracts.
The Connexin 50 (N-term) antibody is typically developed using synthetic peptides or recombinant proteins corresponding to the N-terminal epitope, ensuring specificity for Cx50 over other connexin isoforms. It is employed in techniques like Western blotting, immunohistochemistry, and immunofluorescence to detect Cx50 in tissue samples or cultured cells. Researchers also use it to investigate post-translational modifications, oligomerization, and trafficking dynamics of Cx50. which influence gap junction assembly and function. Validation often includes knockout controls or siRNA-mediated silencing to confirm target specificity. This antibody serves as a key tool in elucidating the pathophysiology of Cx50-related disorders and exploring therapeutic strategies for diseases associated with impaired gap junction communication.
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