| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Palmitoyltransferase ZDHHC6, Transmembrane protein H4, Zinc finger DHHC domain-containing protein 6, DHHC-6, Zinc finger protein 376, ZDHHC6, ZNF376 |
| Entrez GeneID | 64429 |
| WB Predicted band size | 47.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This ZDHHC6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 288-317 amino acids from the C-terminal region of human ZDHHC6. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3-4篇与ZDHHC6抗体相关的文献摘要概览(注：文献为示例性内容，具体信息请以实际检索结果为准)：
1. **文献名称**：*"ZDHHC6-mediated palmitoylation of BAG6 regulates its protein stability and function in ER stress"*
**作者**：Sanders SS, Linder ME
**摘要**：本研究利用ZDHHC6特异性抗体，通过免疫沉淀和Western blot技术，揭示了ZDHHC6通过棕榈酰化修饰BAG6蛋白，调控其在内质网应激中的稳定性及功能，为相关疾病机制提供了新见解。
2. **文献名称**：*"Characterization of ZDHHC6 knockout cell lines reveals its role in protein quality control"*
**作者**：Roth AF, Davis NG
**摘要**：通过构建ZDHHC6基因敲除细胞系并结合ZDHHC6抗体检测，研究发现ZDHHC6缺失导致内质网中错误折叠蛋白积累，表明其在蛋白质质量控制中发挥关键作用。
3. **文献名称**：*"ZDHHC6 antibody validation for immunohistochemical analysis in breast cancer tissues"*
**作者**：Ko PJ, Dixon JE
**摘要**：该研究系统验证了ZDHHC6抗体在乳腺癌组织中的特异性，证实其在免疫组化中的适用性，并发现ZDHHC6表达水平与患者预后显著相关。
4. **文献名称**：*"Subcellular localization of ZDHHC6 and its interaction with HSPA5 in the endoplasmic reticulum"*
**作者**：Fukata Y, Fukata M
**摘要**：通过免疫荧光和共聚焦显微镜技术(使用ZDHHC6抗体)，明确了ZDHHC6在内质网的定位，并发现其与分子伴侣HSPA5的相互作用，提示其在应激反应中的调控机制。
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**注**：以上文献信息为示例，实际文献需通过PubMed、Web of Science等数据库检索确认。若需具体文献DOI或全文链接，请提供更详细的研究背景或关键词。
ZDHHC6 is a member of the DHHC (Asp-His-His-Cys) protein family, which functions as a palmitoyltransferase enzyme. It catalyzes the post-translational addition of palmitate to specific cysteine residues in target proteins, a process known as S-palmitoylation. This modification regulates protein localization, stability, and interactions. ZDHHC6 is primarily localized in the endoplasmic reticulum (ER) and has been implicated in ER-associated degradation (ERAD) and the quality control of membrane proteins. Studies suggest its role in modulating the palmitoylation of proteins like BCL2-associated athanogene 6 (BAG6) and calnexin, influencing cellular stress responses and cancer progression.
Antibodies targeting ZDHHC6 are essential tools for studying its expression, localization, and function. These antibodies are typically developed in hosts like rabbits or mice using immunogenic peptides or recombinant protein fragments. They are validated for applications such as Western blotting, immunofluorescence, and immunohistochemistry. Research using ZDHHC6 antibodies has revealed its dysregulation in cancers (e.g., breast, colorectal) and neurodegenerative diseases, linking altered palmitoylation to pathological mechanisms. However, challenges remain in ensuring antibody specificity due to high homology among DHHC family members. Recent studies emphasize the importance of using knockout controls or complementary techniques to confirm target specificity. Commercial ZDHHC6 antibodies are available from suppliers like Abcam and Santa Cruz Biotechnology, often accompanied by protocols for optimal use in diverse experimental models.
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