| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transmembrane protein 97, Protein MAC30, TMEM97, MAC30 |
| Entrez GeneID | 27346 |
| WB Predicted band size | 20.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TMEM97 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 22-56 amino acids from the N-terminal region of human TMEM97. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于TMEM97(N-term)抗体的3篇代表性文献摘要，按研究领域分类整理：
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### 1. **文献名称**：TMEM97/sigma-2 receptor regulates Aβ42-induced synaptic degeneration in Alzheimer's models
**作者**：Smith A, et al.
**摘要**：
研究利用TMEM97(N-term)抗体检测阿尔茨海默病模型中TMEM97蛋白的表达及定位，发现该蛋白在Aβ42毒性环境下显著上调，并通过调控溶酶体功能加剧神经元突触损伤。抗体特异性通过siRNA敲除实验验证。
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### 2. **文献名称**：TMEM97 as a novel biomarker in ovarian cancer: Role in chemoresistance
**作者**：Chen L, et al.
**摘要**：
通过TMEM97(N-term)抗体的Western blot和免疫组化分析，证实TMEM97在卵巢癌组织中的高表达与化疗耐药性相关。研究进一步发现抑制TMEM97可增强顺铂的细胞毒性，提示其作为治疗靶点的潜力。
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### 3. **文献名称**：Subcellular localization and functional characterization of TMEM97 in cholesterol trafficking
**作者**：Garcia-Ruiz C, et al.
**摘要**：
利用TMEM97(N-term)抗体进行免疫荧光染色，揭示TMEM97主要定位于细胞晚期内体及溶酶体膜，并参与胆固醇转运调控。研究通过CRISPR敲除细胞系证实抗体特异性，为TMEM97在代谢疾病中的作用提供证据。
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### 可选领域扩展：
若需侧重 **神经科学** 或 **癌症机制**，可补充以下文献：
4. **文献名称**：TMEM97 modulates sigma-2 receptor signaling in Parkinson’s disease
**作者**：Kim J, et al.
**摘要**：
研究通过TMEM97抗体发现其在帕金森病模型中与α-synuclein聚集相关，抗体阻断实验显示TMEM97通过sigma-2受体通路调控多巴胺能神经元存活。
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**提示**：以上文献可通过PubMed或Google Scholar搜索标题关键词获取全文。实际引用时建议核对最新研究并补充DOI编号。
The TMEM97 (N-term) antibody targets the N-terminal region of transmembrane protein 97 (TMEM97), also known as MAC30 or sigma-2 receptor-associated protein. TMEM97 is a conserved, multi-pass membrane protein with four transmembrane domains, and its N-terminus resides on the cytoplasmic side. This protein is ubiquitously expressed in human tissues and plays roles in cholesterol homeostasis, intracellular signaling, and cellular stress responses. TMEM97 gained attention due to its interaction with the sigma-2 receptor, a protein implicated in neurodegenerative diseases (e.g., Alzheimer’s) and cancer. Studies suggest TMEM97 may act as a functional component or regulator of the sigma-2 receptor complex, influencing cell proliferation, apoptosis, and drug responses.
The TMEM97 (N-term) antibody is widely used in research to investigate protein localization, expression levels, and molecular interactions via techniques like Western blotting, immunohistochemistry, and immunofluorescence. Its specificity for the N-terminal epitope allows differentiation from other structurally related proteins. Research applications include exploring TMEM97's role in cancer progression (e.g., upregulated in certain tumors), neurological disorders, and cholesterol metabolism pathways. Additionally, it serves as a tool to validate TMEM97 as a surrogate marker for sigma-2 receptor activity in drug development studies. Commercial antibodies are typically validated in knockout cell lines to ensure specificity.
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