| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Vimentin, VIM |
| Entrez GeneID | 7431 |
| WB Predicted band size | 53.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Vimentin antibody is generated from rabbits immunized with human Vimentin recombinant protein. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于Vimentin抗体的代表性文献概览：
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1. **文献名称**: *Vimentin: The chemical and structural basis of tissue-specific localization*
**作者**: Franke WW, et al.
**摘要**: 该研究系统分析了Vimentin抗体在多种哺乳动物组织中的特异性，证实其在间充质细胞中的高表达，并建立了Vimentin作为细胞分化状态的关键标记物，为后续病理诊断提供基础依据。
2. **文献名称**: *Antibody validation guidelines for vimentin immunohistochemistry*
**作者**: Perez-Sanchez C, et al.
**摘要**: 文章提出针对Vimentin抗体的标准化验证流程，强调其在癌症病理学(如肉瘤和转移癌)中的特异性检测需结合多组学数据，以减少假阳性结果。
3. **文献名称**: *Vimentin in circulating tumor cells as a novel metastatic marker*
**作者**: Satelli A, et al.
**摘要**: 研究利用特异性Vimentin抗体检测循环肿瘤细胞(CTC)，发现其表达与癌症患者转移风险显著相关，为液体活检技术提供潜在生物标志物。
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**领域覆盖**：上述文献分别从基础生物学、抗体验证方法学和临床转化角度总结了Vimentin抗体的应用，涉及癌症诊断、细胞分化和实验标准化等方向。如需扩展，可补充EMT(上皮间质转化)相关研究文献。
Vimentin antibodies are essential tools in biomedical research and diagnostics, primarily targeting vimentin, a type III intermediate filament protein. Expressed in mesenchymal cells, vimentin plays a key role in maintaining cytoskeletal integrity, cell migration, and signaling. Its expression is often associated with epithelial-mesenchymal transition (EMT), a process linked to cancer metastasis, fibrosis, and embryonic development.
In research, vimentin antibodies are widely used to study cellular differentiation, EMT, and disease mechanisms. They serve as biomarkers for mesenchymal-derived cancers (e.g., sarcomas, melanomas) and are employed in immunohistochemistry (IHC) to distinguish mesenchymal tumors from epithelial ones. Additionally, these antibodies aid in investigating inflammatory and autoimmune diseases, as vimentin is implicated in conditions like rheumatoid arthritis and systemic lupus erythematosus.
Commercial vimentin antibodies are typically monoclonal (e.g., clones V9. 3B4) or polyclonal, with reactivity across species (human, mouse, rat). Validation includes Western blotting, immunofluorescence, and IHC. However, users must consider cross-reactivity with other intermediate filaments and confirm antibody specificity for their experimental models. Recent studies also explore vimentin's role in viral infections (e.g., SARS-CoV-2) and its extracellular functions, expanding the utility of these antibodies in emerging research fields.
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