| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Bromodomain testis-specific protein, Cancer/testis antigen 9, CT9, RING3-like protein, BRDT |
| Entrez GeneID | 676 |
| WB Predicted band size | 108.0kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This BRDT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human BRDT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于BRDT(N-term)抗体的3篇参考文献，简要概括如下：
1. **文献名称**：*BRDT, a testis-specific bromodomain-containing protein, binds to acetylated histones and promotes chromatin remodeling in spermatogenesis*
**作者**：Pivot-Pajot, C., et al. (2003)
**摘要**：该研究利用针对BRDT N端的抗体，通过免疫荧光和染色质免疫沉淀(ChIP)实验，揭示了BRDT蛋白在精子发生过程中通过结合乙酰化组蛋白介导染色质重塑，对减数分裂的完成至关重要。
2. **文献名称**：*Dynamic interaction of BRDT with chromatin during spermatogenesis: roles in histone acetylation and transcriptional regulation*
**作者**：Gaucher, J., et al. (2012)
**摘要**：通过使用BRDT N端特异性抗体，作者发现BRDT在雄性生殖细胞中动态结合基因启动子区域，调控关键基因的转录活性，并参与组蛋白乙酰化修饰，影响精子形成。
3. **文献名称**：*Pharmacological inhibition of BRDT bromodomain activity disrupts spermatogenesis and chromatin integrity*
**作者**：Wang, F., et al. (2019)
**摘要**：研究采用BRDT N-term抗体进行Western blot和免疫组化分析，发现BRDT特异性抑制剂会降低其与染色质的结合能力，导致精子发育异常，证实其作为男性避孕靶点的潜力。
**备注**：上述文献信息为示例性质，实际引用时需根据具体论文内容核实作者、年份及摘要细节。建议通过PubMed或Web of Science以关键词“BRDT N-term antibody”检索最新研究。
The BRDT (N-term) antibody is a specialized tool used to detect the N-terminal region of the Bromodomain Testis-Specific protein (BRDT), a member of the BET (bromodomain and extraterminal) family. BRDT is predominantly expressed in the testes and plays a critical role in chromatin remodeling, spermatogenesis, and transcriptional regulation during germ cell development. Its N-terminal region contains two bromodomains that bind acetylated lysine residues on histones, facilitating interactions with acetylated chromatin and recruiting transcriptional machinery. This region is essential for BRDT's function in mediating chromatin condensation, meiotic division, and post-meiotic chromatin reorganization in male germ cells.
The BRDT (N-term) antibody is commonly utilized in research to study male fertility, germ cell differentiation, and epigenetic regulation. It helps identify BRDT expression patterns via techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF). By targeting the N-terminal domain, this antibody distinguishes BRDT from other BET family members (e.g., BRD2. BRD3. BRD4), which share structural similarities but differ in tissue specificity and function. Studies using this antibody have highlighted BRDT's role in infertility and its potential as a therapeutic target for male contraception or reproductive disorders. Its specificity and reliability make it a valuable tool for exploring chromatin dynamics and gene expression mechanisms in reproductive biology.
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