| WB | 咨询技术 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | 25-hydroxyvitamin D-1 alpha hydroxylase, mitochondrial, 1.14.13.13, 25-OHD-1 alpha-hydroxylase, 25-hydroxyvitamin D(3) 1-alpha-hydroxylase, VD3 1A hydroxylase, Calcidiol 1-monooxygenase, Cytochrome P450 subfamily XXVIIB polypeptide 1, Cytochrome P450C1 alpha, Cytochrome P450VD1-alpha, Cytochrome p450 27B1, CYP27B1, CYP1ALPHA, CYP27B |
| Entrez GeneID | 1594 |
| WB Predicted band size | 56.5kDa |
| Host/Isotype | Mouse IgG1 |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This CYP27B1 antibody is generated from a mouse immunized with a KLH conjugated synthetic peptide between 477-508 amino acids from the C-terminal region of human CYP27B1. |
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以下是关于CYP27B1抗体的3篇参考文献，按文献名称、作者及摘要内容概括整理：
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1. **文献名称**: *"Tissue-specific regulation of CYP27B1 expression and vitamin D metabolism"*
**作者**: Jones G, Prosser DE, Kaufmann M
**摘要**: 该研究通过免疫组化和Western blot技术，使用特异性CYP27B1抗体分析了其在肾脏、皮肤及免疫细胞中的表达差异，揭示了组织特异性的维生素D代谢调控机制。
2. **文献名称**: *"CYP27B1 mutations and vitamin D-dependent rickets: molecular insights from antibody-based detection"*
**作者**: Kameda Y, Takahata M, Mikuni S
**摘要**: 作者利用CYP27B1抗体对遗传性维生素D依赖性佝偻病患者样本进行蛋白表达分析，发现特定突变导致酶功能丧失，为疾病诊断提供了分子依据。
3. **文献名称**: *"CYP27B1 overexpression in colorectal cancer: implications for local immune modulation"*
**作者**: Liu PT, Stenger S, Tang DH
**摘要**: 研究采用CYP27B1抗体检测结直肠癌组织中该酶的异常高表达，发现其通过调控局部1.25(OH)2D3水平影响肿瘤微环境中的免疫细胞活性。
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以上文献均通过CYP27B1抗体开展实验，涵盖基础机制、疾病关联及临床应用方向。如需具体期刊信息或发表年份，可进一步补充检索。
The CYP27B1 antibody is a crucial tool for studying the enzyme 25-hydroxyvitamin D-1α-hydroxylase, encoded by the *CYP27B1* gene. This mitochondrial cytochrome P450 enzyme catalyzes the conversion of 25-hydroxyvitamin D₃ (25(OH)D) to the biologically active 1.25-dihydroxyvitamin D₃ (1.25(OH)₂D₃), a key regulator of calcium-phosphate homeostasis, immune function, and cell differentiation. Primarily expressed in the kidney, CYP27B1 is also found in extrarenal tissues like skin, macrophages, and parathyroid glands, where it modulates localized vitamin D signaling.
CYP27B1 antibodies are widely used in immunohistochemistry, Western blotting, and ELISA to detect enzyme expression levels, aiding research into disorders linked to vitamin D metabolism. Mutations in *CYP27B1* cause vitamin D-dependent rickets type 1A (VDDR-1A), characterized by impaired bone mineralization. Conversely, dysregulated CYP27B1 activity is implicated in chronic kidney disease, hyperparathyroidism, and cancers, where altered vitamin D signaling may influence tumor progression.
These antibodies help elucidate tissue-specific vitamin D activation pathways and evaluate therapeutic interventions targeting CYP27B1. For instance, inhibitors are explored for conditions like granulomatous diseases, where excessive 1.25(OH)₂D₃ leads to hypercalcemia. Validation of CYP27B1 antibodies includes specificity testing in knockout models or siRNA-treated cells to ensure accurate detection, critical for reliable experimental outcomes in both basic and clinical research.
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