| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Zinc finger and BTB domain-containing protein 8A, BTB/POZ and zinc-finger domain-containing factor, BTB/POZ and zinc-finger domains factor on chromosome 1, BOZ-F1, ZBTB8A, BOZF1 |
| Entrez GeneID | 653121 |
| WB Predicted band size | 50.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ZBTB8A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 205-232 amino acids from the Central region of human ZBTB8A. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ZBTB8A抗体的模拟参考文献示例(内容为虚构，仅作格式参考)：
1. **文献名称**: "ZBTB8A as a Tumor Suppressor in Breast Cancer: Functional Characterization and Antibody Validation"
**作者**: Li, X., et al.
**摘要**: 本研究利用特异性ZBTB8A抗体通过免疫组化和Western blot分析，发现ZBTB8A在乳腺癌组织中低表达，并证实其通过抑制AKT/mTOR信号通路抑制肿瘤增殖。
2. **文献名称**: "Epigenetic Silencing of ZBTB8A in Colorectal Cancer: Implications for Diagnostic Biomarker Development"
**作者**: Martinez, R., et al.
**摘要**: 通过ZBTB8A抗体检测结直肠癌样本，发现启动子甲基化导致其表达缺失，与患者不良预后相关，提示其作为潜在表观遗传标志物的价值。
3. **文献名称**: "ZBTB8A Regulates MYCN Stability in Neuroblastoma: Insights from CRISPR/Cas9 and Antibody-Based Approaches"
**作者**: Chen, H., et al.
**摘要**: 结合CRISPR/Cas9基因编辑和ZBTB8A抗体免疫沉淀技术，揭示ZBTB8A通过泛素化降解通路调控MYCN蛋白稳定性，影响神经母细胞瘤进展。
4. **文献名称**: "Antibody-Based Profiling of ZBTB8A in Non-Small Cell Lung Cancer: Correlation with Immune Microenvironment"
**作者**: Wang, Y., et al.
**摘要**: 使用ZBTB8A抗体对肺癌组织进行染色，发现其高表达与CD8+ T细胞浸润正相关，提示其在肿瘤免疫微环境中的调控作用及预后意义。
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**注意**：以上文献信息均为模拟生成，实际研究中请通过PubMed、Web of Science等数据库检索真实文献。如需进一步协助文献检索，请提供更多具体需求。
The ZBTB8A antibody is a research tool designed to detect and study the ZBTB8A protein, a member of the zinc finger and BTB domain-containing (ZBTB) family. ZBTB8A, also known as Zinc Finger and BTB Domain-Containing Protein 8A, functions as a transcriptional regulator implicated in cellular processes such as cell cycle control, apoptosis, and tumor suppression. Structurally, it contains an N-terminal BTB domain for protein-protein interactions and C-terminal zinc finger motifs for DNA binding. Dysregulation of ZBTB8A has been linked to cancer progression, particularly in breast and prostate cancers, where it may act as a tumor suppressor or oncogene depending on context.
The antibody is commonly used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to assess ZBTB8A expression levels, subcellular localization, and interactions in cell lines or tissue samples. Commercial ZBTB8A antibodies are typically monoclonal or polyclonal, raised against specific epitopes (e.g., human ZBTB8A peptides), and validated for specificity through knockdown/knockout controls. Researchers employ these antibodies to explore ZBTB8A’s role in cancer biology, its interaction with pathways like p53 or Myc, and its potential as a therapeutic target. Variability in antibody performance across applications underscores the need for thorough validation to ensure reproducibility in experimental models.
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