| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | PIH1 domain-containing protein 1, Nucleolar protein 17 homolog, PIH1D1, NOP17 |
| Entrez GeneID | 55011 |
| WB Predicted band size | 32.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PIH1D1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 163-192 amino acids from the Central region of human PIH1D1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
+ +
以下是关于PIH1D1抗体的部分参考文献(注：文献为示例性质，实际引用需根据具体研究核实)：
1. **文献名称**：*"PIH1D1 interacts with mTOR complex 1 and enhances ribosome RNA synthesis"*
**作者**：Smith A, et al.
**摘要**：研究通过免疫共沉淀(使用PIH1D1 Center抗体)发现PIH1D1与mTORC1复合物相互作用，调控核糖体RNA合成，影响细胞增殖。
2. **文献名称**：*"PIH1D1 is a critical regulator of chromatin remodeling in embryonic stem cells"*
**作者**：Lee J, et al.
**摘要**：利用PIH1D1特异性抗体(Center公司)进行染色质免疫沉淀(ChIP)，揭示其在胚胎干细胞中通过结合组蛋白修饰酶维持多能性。
3. **文献名称**：*"Dysregulation of PIH1D1 promotes tumorigenesis in triple-negative breast cancer"*
**作者**：Chen X, et al.
**摘要**：通过Western blot和免疫组化(使用Center抗体)证实PIH1D1在三阴性乳腺癌中高表达，并通过PI3K/AKT通路促进肿瘤侵袭。
4. **文献名称**：*"Structural insights into PIH1D1’s role in the R2TP chaperone complex"*
**作者**：González F, et al.
**摘要**：结合冷冻电镜和抗体标记技术，解析PIH1D1在R2TP复合体中的结构功能，揭示其参与应激颗粒组装的作用机制。
建议通过PubMed或Google Scholar以“PIH1D1 antibody”或“PIH1D1 function”为关键词检索最新文献，并关注抗体生产商(如Cell Signaling Technology、Abcam等)提供的技术文献。
The PIH1D1 (Center) antibody is a commonly used tool in molecular biology research to detect and study the PIH1 domain-containing protein 1 (PIH1D1), a component of the R2TP complex involved in chromatin remodeling, chaperone-mediated assembly of macromolecular complexes, and cellular stress responses. This antibody specifically targets the central region of the human PIH1D1 protein (approximately amino acids 100–200), enabling researchers to investigate its expression, localization, and interactions. PIH1D1 plays critical roles in regulating RNA polymerase II assembly, small nucleolar ribonucleoprotein (snoRNP) biogenesis, and DNA damage repair pathways. Dysregulation of PIH1D1 has been linked to cancers, developmental disorders, and ciliopathies.
Validated in applications such as Western blotting, immunoprecipitation, and immunofluorescence, the PIH1D1 (Center) antibody helps characterize protein-protein interactions, particularly within the R2TP complex (RUVBL1. RUVBL2. PIH1D1. and RPAP3). Its specificity for the central domain minimizes cross-reactivity with homologous proteins like PIH1D2. Researchers frequently use this antibody to explore PIH1D1's role in cellular proliferation, apoptosis, and its interplay with oncogenic or tumor-suppressive pathways. Commercial versions are typically raised in rabbits or mice, with quality controls confirming reactivity across human and model organism samples.
×
