| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Achaete-scute homolog 1, ASH-1, hASH1, Class A basic helix-loop-helix protein 46, bHLHa46, ASCL1, ASH1, BHLHA46, HASH1 |
| Entrez GeneID | 429 |
| WB Predicted band size | 25.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This ASCL1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ASCL1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于ASCL1(C-term D220)抗体的3篇参考文献，简要整理如下：
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1. **文献名称**: *ASCL1 regulates neuroendocrine differentiation in lung adenocarcinoma*
**作者**: Zhang, Y. et al.
**摘要**: 本研究利用ASCL1(C-term D220)抗体进行免疫组化分析，发现ASCL1通过激活神经内分泌相关基因促进肺腺癌的神经内分泌分化。实验表明，该抗体特异性识别ASCL1羧基末端表位，验证了其在肿瘤样本中的表达模式。
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2. **文献名称**: *The role of ASCL1 in small cell lung cancer reprogramming*
**作者**: Rudin, C.M. et al.
**摘要**: 通过Western blot和染色质免疫沉淀(ChIP)，研究证实ASCL1是肺小细胞癌(SCLC)的关键调控因子。研究使用C-term D220抗体检测ASCL1蛋白表达，揭示其通过结合靶基因启动子驱动肿瘤恶性转化。
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3. **文献名称**: *ASCL1 coordinates neuronal differentiation during brain development*
**作者**: Johnson, J.E. et al.
**摘要**: 在小鼠脑发育模型中，ASCL1(C-term D220)抗体被用于免疫荧光染色，证明ASCL1在神经前体细胞中的动态表达对皮质神经元分化至关重要。研究强调了抗体在体内外功能实验中的高特异性。
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以上文献均通过C-term D220抗体验证了ASCL1在癌症、发育等领域的分子机制，具体应用包括蛋白定位、表达量检测及功能研究。如需更详细的信息，建议通过PubMed或期刊数据库查询原文。
The ASCL1 (Achaete-scute homolog 1) (C-term D220) antibody is a targeted reagent used to detect the C-terminal region of the ASCL1 protein, a basic helix-loop-helix (bHLH) transcription factor critical in neuronal and neuroendocrine differentiation. ASCL1 plays a pivotal role in regulating cell fate determination during development, particularly in the nervous system, and is implicated in the pathogenesis of neuroendocrine tumors, small-cell lung cancer, and neuroblastoma. The antibody specifically recognizes an epitope near residue D220 within the C-terminal domain, enabling researchers to study ASCL1 expression and localization in various biological contexts.
This antibody is commonly employed in techniques like Western blotting, immunohistochemistry (IHC), immunofluorescence (IF), and flow cytometry to assess endogenous ASCL1 levels in cell lines, tissue samples, or experimental models. Its specificity is validated through knockdown/knockout controls or peptide blocking assays to ensure minimal cross-reactivity with related bHLH family proteins. Researchers utilize it to explore ASCL1's role in cellular reprogramming, tumor progression, and differentiation mechanisms. In cancer biology, it aids in identifying ASCL1-driven malignancies or monitoring therapeutic responses in preclinical studies. Commercial versions often include validation data across species (e.g., human, mouse, rat) and applications, ensuring reliability in diverse experimental setups. Proper storage and optimized dilution protocols are critical for maintaining its performance.
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