| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nuclear receptor subfamily 0 group B member 1, DSS-AHC critical region on the X chromosome protein 1, Nuclear receptor DAX-1, NR0B1, AHC, DAX1 |
| Entrez GeneID | 190 |
| WB Predicted band size | 51.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This DAX1 (NR0B1) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 4-34 amino acids from the N-terminal region of human DAX1 (NR0B1). |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于DAX1 (NR0B1) N端抗体的参考文献概览：
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1. **"DAX1 mutations in adrenal hypoplasia congenita and hypogonadotropic hypogonadism"**
*Authors: Muscatelli F, et al. (1994)*
摘要：研究首次克隆DAX1基因并制备其N端特异性抗体，通过免疫组化验证该抗体在肾上腺和性腺组织中的特异性表达，揭示了DAX1突变与先天性肾上腺发育不良的关联。
2. **"The nuclear receptor DAX1 inhibits the transcriptional activity of steroidogenic factor 1 (SF1)"**
*Authors: Lalli E, et al. (1998)*
摘要：利用针对DAX1 N端结构域的多克隆抗体(抗原为N端1-100氨基酸片段)，通过免疫共沉淀和Western blot证明DAX1与SF1的相互作用，阐明其转录抑制功能。
3. **"Tissue-specific roles of DAX1 (NR0B1) in adrenal development and function"**
*Authors: Kim AC, et al. (2009)*
摘要：通过N端特异性抗体进行免疫荧光和染色质免疫沉淀(ChIP)，揭示DAX1在肾上腺皮质祖细胞中的时空表达模式及其在类固醇生成调控中的作用。
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以上文献聚焦于DAX1抗体的开发验证及其在基因功能、疾病机制研究中的应用，涵盖肾上腺发育、转录调控等方向。实际引用时建议核对文献年份及期刊来源准确性。
The DAX1 (Dosage-sensitive sex reversal, adrenal hypoplasia critical region, on chromosome X, gene 1) protein, encoded by the *NR0B1* gene, is a nuclear receptor family member that plays crucial roles in adrenal gland development, steroidogenesis, and reproductive biology. Unlike classical nuclear receptors, DAX1 lacks a DNA-binding domain but contains a unique N-terminal domain critical for its transcriptional repressor activity. The DAX1 (NR0B1) (N-term) antibody specifically targets the N-terminal region of the protein, enabling researchers to study its expression, localization, and interactions in developmental and pathological contexts.
DAX1 is essential for adrenal cortex formation and regulates the hypothalamic-pituitary-gonadal axis by repressing steroidogenic factor 1 (SF-1)-mediated transcription. Mutations in *NR0B1* cause X-linked adrenal hypoplasia congenita (AHC) and hypogonadotropic hypogonadism. The antibody is widely used in applications like Western blotting, immunohistochemistry, and immunofluorescence to investigate DAX1's role in endocrine disorders, tumorigenesis (e.g., adrenocortical carcinoma, testicular germ cell tumors), and sex determination pathways. Its specificity for the N-terminal region ensures detection of full-length DAX1 isoforms while avoiding cross-reactivity with truncated variants or related proteins. Validation in knockout models or siRNA-treated cells is recommended to confirm antibody reliability in experimental systems.
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