Rabbit Polyclonal Vinculin Antibody

+ +

•  

   
     All lanes : Anti-Vinculin at 1:2000 dilution Lane 1: human skeletal muscle lysate Lane 2: A431 whole cell lysate Lane 3: Hela whole cell lysate Lane 4: mouse kidney lysate Lane 5: PC-12 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 117 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

•  

   
     Overlay histogram showing NIH/3T3 cells stained with P34038(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34038,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

•  

   
     P34038 staining Vinculin in mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

Vinculin is a cytoskeletal protein crucial for cell-cell and cell-matrix adhesion, primarily localized to focal adhesions and adherens junctions. It acts as a scaffolding molecule, linking transmembrane adhesion receptors (e.g., integrins, cadherins) to the actin cytoskeleton, thereby regulating cell adhesion, migration, and mechanical signaling. Vinculin undergoes conformational changes to switch between inactive (auto-inhibited) and active states, enabling its interaction with binding partners like talin, α-actinin, and F-actin. Its role in maintaining cellular integrity and mechanotransduction makes it vital in processes such as embryogenesis, tissue homeostasis, and wound healing.

Vinculin antibodies are essential tools for studying its expression, localization, and function in cellular and pathological contexts. Commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry, these antibodies help detect vinculin’s presence in tissues or cultured cells. Specific monoclonal antibodies (e.g., clone hVIN-1) target epitopes in the vinculin head or tail domains, aiding in distinguishing its active/inactive states. Validation methods, including knockout cell lines or siRNA knockdown, ensure antibody specificity, minimizing cross-reactivity with homologous proteins like metavinculin.

Dysregulation of vinculin is implicated in cancer metastasis, cardiovascular diseases, and neurological disorders. Antibodies against vinculin thus contribute to research on cell adhesion dynamics, disease mechanisms, and potential therapeutic targets.