Rabbit Polyclonal betaTubulin Antibody

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     All lanes : Anti-beta Tubulin at 1:2000 dilution Lane 1: C2C12 whole cell lysate Lane 2: L6 whole cell lysate Lane 3: mouse brain lysate Lane 4: mouse spleen whole cell lysate Lane 5: mouse thymus lysate Lane 6: NIH/3T3 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 50 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing C2C12 cells stained with P34036(green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P34036,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG1 (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P34036 staining beta Tubulin in mouse thymus tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

Beta-tubulin, a crucial component of microtubules, is a highly conserved cytoskeletal protein involved in vital cellular processes such as mitosis, intracellular transport, and maintenance of cell shape. As part of the tubulin superfamily, it forms heterodimers with alpha-tubulin, polymerizing into dynamic microtubule networks. Multiple beta-tubulin isotypes exist (e.g., βI, βIII, βIV), with tissue-specific expression patterns and functional specializations. For instance, βIII-tubulin is enriched in neurons and serves as a neuronal marker, while βI is ubiquitously expressed.

Beta-tubulin antibodies are essential tools in biomedical research, widely used as loading controls in Western blotting and immunohistochemistry due to beta-tubulin's consistent expression across most cell types. These antibodies typically target conserved epitopes in the C-terminal region, enabling broad species reactivity. Monoclonal antibodies (e.g., AA2. D71G3) offer high specificity, whereas polyclonal antibodies may detect multiple isotypes. Researchers often validate them using knockout cell lines or siRNA knockdown to confirm specificity. Beyond housekeeping applications, beta-tubulin antibodies help study microtubule dynamics, drug mechanisms (e.g., taxanes, vinca alkaloids), and pathological conditions like neurodegenerative diseases and cancer. Commercial availability from major suppliers and standardized validation protocols have cemented their role as indispensable reagents in cellular and molecular biology studies.