Rabbit Polyclonal RASIP1 Antibody

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     All lanes : Anti-RASIP1 Antibody (Center) at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: HUVEC whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: K562 whole cell lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 103 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Western blot analysis of RASIP1 Antibody (Center) (Cat. #P33860) in K562 cell line lysates (35ug/lane). RASIP1 (arrow) was detected using the purified Pab.    

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     Overlay histogram showing K562 cells stained with P33860 (green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33860,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(OH191631) at 1/200 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     Formalin-fixed and paraffin-embedded human brain tissue reacted with RASIP1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.    

**Background of RASIP1 Antibody**

RASIP1 (Ras Interacting Protein 1) is a cytoplasmic protein that regulates endothelial cell morphogenesis, vascular lumen formation, and vascular stability by interacting with Rho GTPases and modulating cell adhesion and cytoskeletal dynamics. It plays a critical role in angiogenesis and maintaining vascular integrity by suppressing MAPK signaling and stabilizing cell-cell junctions.

RASIP1 antibodies are immunological tools designed to detect and study RASIP1 expression, localization, and function in various biological contexts. These antibodies are commonly used in techniques like Western blotting, immunofluorescence, and immunohistochemistry to investigate RASIP1's role in endothelial cell behavior, vascular development, and disease models, such as cancer or cardiovascular disorders. Studies utilizing RASIP1 antibodies have highlighted its importance in tumor angiogenesis, where its dysregulation correlates with abnormal vessel formation and metastasis.

Developed in multiple host species (e.g., rabbit, mouse), RASIP1 antibodies are validated for specificity and affinity, often targeting conserved regions of the protein. Their applications extend to both basic research and preclinical studies, aiding in the exploration of RASIP1 as a potential therapeutic target for diseases involving aberrant vascular remodeling.