| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | N-alpha-acetyltransferase 30, N-acetyltransferase 12, N-acetyltransferase MAK3 homolog, NatC catalytic subunit, NAA30, C14orf35, MAK3, NAT12 |
| Entrez GeneID | 122830 |
| WB Predicted band size | 39.3kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This NAT12 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 315-343 amino acids from the C-terminal region of human NAT12. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇关于NAT12抗体的模拟参考文献示例(注：部分文献信息为虚构，供参考格式)：
1. **文献名称**: *NAT12-mediated protein acetylation regulates cellular stress response*
**作者**: Li, X.; Zhang, Y.; Wang, H.
**摘要**: 该研究报道了NAT12(N-乙酰转移酶12)通过乙酰化特定底物蛋白参与细胞应激反应的分子机制，开发了一种高特异性NAT12抗体用于检测其在氧化应激模型中的表达变化。
2. **文献名称**: *Development of a monoclonal antibody against NAT12 for cancer biomarker screening*
**作者**: Tanaka, K.; et al.
**摘要**: 文章描述了一种新型NAT12单克隆抗体的制备及验证，该抗体在多种癌症组织(如乳腺癌和肺癌)中显示出高灵敏度和特异性，可用于临床样本的免疫组化检测。
3. **文献名称**: *NAT12 interacts with p53: Evidence from co-immunoprecipitation using specific antibodies*
**作者**: Chen, L.; Zhou, M.; Wei, J.
**摘要**: 通过免疫共沉淀(Co-IP)实验结合NAT12抗体，研究发现NAT12与肿瘤抑制蛋白p53存在直接相互作用，提示其在DNA损伤修复通路中的潜在调控作用。
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**备注**：以上文献为示例性内容，实际研究中请通过PubMed或Web of Science等数据库检索真实文献(关键词：NAT12 antibody、N-acetyltransferase 12等)。
**Background of NAT12 Antibody**
The NAT12 antibody targets NAT12 (N-acetyltransferase 12), an enzyme encoded by the *NAT12* gene, which belongs to the GNAT (GCN5-related N-acetyltransferase) family. NAT12 catalyzes the acetylation of α-tubulin at lysine 40 (αTAT1 activity), a post-translational modification critical for regulating microtubule stability, dynamics, and interactions with motor proteins. This acetylation is implicated in cellular processes such as cell motility, intracellular trafficking, and cilia assembly.
NAT12's role extends to maintaining cytoskeletal integrity and influencing cellular responses to mechanical stress. Dysregulation of α-tubulin acetylation has been linked to pathologies, including neurodegenerative diseases, cardiovascular disorders, and cancer metastasis. The NAT12 antibody is widely used in research to study microtubule biology, assess acetylation levels in disease models, and explore therapeutic targets.
As a tool, the antibody enables detection of NAT12 expression and α-tubulin acetylation via techniques like Western blot, immunofluorescence, and immunohistochemistry. Its specificity helps distinguish acetylated microtubules from other post-translationally modified forms, providing insights into cytoskeletal regulation. Recent studies also highlight NAT12's potential in modulating immune cell function and mechanotransduction pathways, underscoring its relevance in both basic and translational research.
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