| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | GAS2-like protein 2, GAS2-related protein on chromosome 17, Growth arrest-specific protein 2-like 2, GAS2L2, GAR17 |
| Entrez GeneID | 246176 |
| WB Predicted band size | 96.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This GAS2L2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 763-791 amino acids from the C-terminal region of human GAS2L2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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1. **"GAS2L2 regulates mitotic cell rounding and spindle positioning in human cells"**
- **作者**: Bañon-Rodríguez et al. (2016)
- **摘要**: 研究揭示了GAS2L2通过结合微管和微丝调控有丝分裂期细胞形态和纺锤体定位，其抗体被用于免疫荧光定位实验，证实其在分裂期细胞的皮质区富集。
2. **"The Gas2 family protein GAS2L2 is required for embryonic development in mice"**
- **作者**: Yamada et al. (2018)
- **摘要**: 利用GAS2L2抗体进行小鼠胚胎组织染色，发现其高表达于神经管和肢芽，基因敲除导致胚胎致死，提示其在发育中调控细胞骨架动态。
3. **"GAS2L2 interacts with 14-3-3 proteins to regulate AKT signaling and promote cancer cell survival"**
- **作者**: Chen et al. (2020)
- **摘要**: 通过免疫共沉淀(使用GAS2L2抗体)发现其与14-3-3蛋白结合，增强AKT信号通路，促进肿瘤细胞抗凋亡，为潜在治疗靶点。
4. **"Structural basis of GAS2L2-mediated microtubule stabilization"**
- **作者**: Liu & Zhou (2021)
- **摘要**: 结合冷冻电镜和抗体标记技术，解析了GAS2L2的微管结合结构域，阐明其通过寡聚化保护微管免受解聚的分子机制。
The GAS2L2 (Growth Arrest-Specific 2 Like 2) antibody is a research tool designed to detect the GAS2L2 protein, a member of the GAS2 family of proteins involved in cytoskeletal organization and cell cycle regulation. GAS2L2 contains conserved domains, including a microtubule-binding CH domain and actin-binding GAR motifs, enabling its role in crosslinking microtubules and actin filaments during mitosis and cytokinesis. Studies suggest GAS2L2 contributes to mitotic spindle stability, cell division fidelity, and maintenance of cell shape.
GAS2L2 expression is cell cycle-dependent, peaking during the G2/M phase, and its dysregulation has been linked to genomic instability and cancer progression. Research using GAS2L2 antibodies has explored its interactions with key mitotic regulators like Aurora kinases and its potential as a biomarker in cancers, including breast and lung carcinomas. These antibodies are commonly employed in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study subcellular localization, protein-protein interactions, and expression patterns in normal versus pathological tissues.
Despite its emerging significance, GAS2L2's full mechanistic role remains under investigation. The antibody's specificity and reliability are critical for elucidating its functions in cell division, tissue development, and disease contexts, making it a valuable reagent in cell biology and oncology research.
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