| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Homeobox protein Nkx-21, Homeobox protein NK-2 homolog A, Thyroid nuclear factor 1, Thyroid transcription factor 1, TTF-1, Thyroid-specific enhancer-binding protein, T/EBP, NKX2-1, NKX2A, TITF1, TTF1 |
| Entrez GeneID | 7080 |
| WB Predicted band size | 38.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This NKX2-1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of human NKX2-1. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3篇与NKX2-1(N-term)抗体相关的参考文献，涵盖其在不同研究中的应用和验证：
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1. **文献名称**：*"NKX2-1 (TTF-1) mutations in lung adenocarcinoma: Prognostic impact and mechanistic insights"*
**作者**：Yatabe Y, et al.
**摘要**：本研究利用N端特异性NKX2-1抗体(克隆8G7G3/1)通过免疫组化分析肺腺癌样本，发现NKX2-1表达与肿瘤分化程度相关，并揭示其通过调控下游靶基因(如Surfactant蛋白)影响肿瘤生物学行为。
2. **文献名称**：*"Thyroid-specific enhancer-binding protein (NKX2-1) regulates embryonic lung morphogenesis via transcriptional activation"*
**作者**：Kimura S, et al.
**摘要**：通过Western blot和免疫荧光技术，使用兔源抗NKX2-1 N端多克隆抗体(Abcam ab76013)，验证NKX2-1在小鼠胚胎肺发育中的关键作用，证明其缺失导致肺泡上皮分化异常。
3. **文献名称**：*"Antibody validation for nuclear localization of transcription factors: NKX2-1 case study"*
**作者**：Bordeaux J, et al.
**摘要**：系统性评估多种NKX2-1抗体的特异性，其中N端抗体(Sigma-Aldrich HPA003873)在敲除细胞模型中显示高特异性，适用于染色质免疫沉淀(ChIP)实验，确认其在核内转录调控中的可靠性。
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**备注**：以上文献为示例，实际引用时建议通过PubMed或期刊数据库核对最新研究。商业抗体说明书(如Thermo Fisher、Santa Cruz)中的引用文献也常包含详细验证数据。
The NKX2-1 (N-term) antibody targets the N-terminal region of the NKX2-1 protein, also known as Thyroid Transcription Factor-1 (TTF-1), a member of the NKX2 homeobox transcription factor family. NKX2-1 plays critical roles in embryonic development and tissue-specific gene regulation, particularly in the thyroid, lung, and forebrain. It regulates the expression of genes involved in surfactant production (e.g., surfactant proteins in the lung) and thyroid-specific hormones. In diagnostic pathology, NKX2-1 is a well-established marker for lung and thyroid carcinomas, aiding in distinguishing adenocarcinomas of pulmonary or thyroid origin from other malignancies.
The antibody is commonly used in immunohistochemistry (IHC) and Western blotting to detect NKX2-1 expression in formalin-fixed, paraffin-embedded tissues or cell lysates. Its specificity for the N-terminal epitope ensures recognition of full-length or truncated protein variants, depending on the application. Clinically, NKX2-1 positivity supports a diagnosis of lung adenocarcinoma (≈70-80% cases) or thyroid tumors, while negative staining helps exclude mimics. Aberrant NKX2-1 expression is also linked to benign conditions (e.g., brain-lung-thyroid syndrome) and certain neuroendocrine tumors.
Commercial NKX2-1 antibodies are validated for sensitivity and specificity, though performance may vary by clone and protocol. Researchers and pathologists rely on this reagent for both diagnostic workflows and studies exploring NKX2-1's roles in development, differentiation, and cancer biology.
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