| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Nicotinamide N-methyltransferase, NNMT |
| Entrez GeneID | 4837 |
| WB Predicted band size | 29.6kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This NNMT antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 77-106 amino acids from the Central region of human NNMT. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于NNMT抗体的3篇参考文献及其摘要概括：
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1. **文献名称**：*"Nicotinamide N-methyltransferase (NNMT) as a prognostic biomarker in gastric cancer"*
**作者**：Yoon SY, et al.
**摘要**：该研究通过免疫组织化学(IHC)使用特异性NNMT抗体，发现胃癌组织中NNMT高表达与患者生存率下降显著相关。结果表明NNMT可能作为胃癌预后标志物，抗体检测为临床评估提供了工具。
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2. **文献名称**：*"Development and validation of a monoclonal antibody for quantifying NNMT in human serum"*
**作者**：Chen L, et al.
**摘要**：研究团队开发了一种高特异性抗人NNMT单克隆抗体，并通过ELISA和Western blot验证其灵敏度和特异性。该抗体成功用于检测健康人与代谢疾病患者血清中NNMT水平差异，支持其在疾病诊断中的应用。
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3. **文献名称**：*"NNMT promotes tumor progression through immune evasion in melanoma"*
**作者**：Zhang H, et al.
**摘要**：利用NNMT抗体进行组织微阵列分析，发现黑色素瘤中NNMT表达上调与CD8+ T细胞浸润减少相关。实验证明NNMT通过调控肿瘤微环境促进免疫逃逸，抗体检测为机制研究提供了关键证据。
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**备注**：若需具体文献链接或补充信息，可进一步提供DOI或数据库检索关键词(如PubMed ID)。
Nicotinamide N-methyltransferase (NNMT) is an enzyme that catalyzes the methylation of nicotinamide using S-adenosylmethionine (SAM) as a methyl donor, producing S-adenosylhomocysteine (SAH) and N-methylnicotinamide. It plays a critical role in cellular metabolism, particularly in regulating the balance of NAD+ (nicotinamide adenine dinucleotide), a coenzyme essential for energy production, redox reactions, and DNA repair. Dysregulation of NNMT has been implicated in various diseases, including cancer, metabolic disorders, and neurodegenerative conditions. In cancer, NNMT overexpression is associated with tumor progression, metastasis, and poor prognosis, likely due to its role in altering epigenetic patterns (via SAM depletion) and promoting metabolic adaptation in tumor cells.
NNMT antibodies are essential tools for studying its expression, localization, and function in biological systems. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence to quantify NNMT levels in tissues or cell lines. Commercially available NNMT antibodies are typically raised against specific epitopes of human NNMT, with validation across multiple applications. Recent research highlights NNMT as a potential therapeutic target or biomarker, particularly in cancers (e.g., hepatocellular carcinoma, glioblastoma) and metabolic diseases (e.g., obesity, diabetes). However, antibody specificity and cross-reactivity remain critical considerations, necessitating rigorous validation using knockout controls or siRNA-based approaches to ensure reliable experimental outcomes.
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