| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein kinase N3, Protein kinase PKN-beta, Protein-kinase C-related kinase 3, Pkn3, Pknbeta |
| Entrez GeneID | 263803 |
| WB Predicted band size | 97.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Pkn3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-30 amino acids from the N-terminal region of mouse Pkn3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于Mouse Pkn3 (N-term)抗体的参考文献示例(注：以下内容为模拟生成，实际文献需根据具体数据库查询)：
1. **"Characterization of a Novel Anti-Mouse Pkn3 (N-terminal) Antibody for Immunoblotting Applications"**
*作者：Smith J, et al.*
摘要：本研究开发并验证了一种针对小鼠Pkn3蛋白N端区域的高特异性多克隆抗体。通过Western blot和免疫荧光实验证实了其在多种小鼠组织中的反应性，并探讨了Pkn3在细胞增殖调控中的作用。
2. **"Pkn3 Kinase Activity in Mouse Embryonic Development: Insights from N-terminal Specific Antibody Staining"**
*作者：Lee S, et al.*
摘要：利用N端特异性Pkn3抗体，研究者揭示了Pkn3在小鼠胚胎发育中的时空表达模式，发现其通过调控Akt/mTOR信号通路参与神经管闭合和器官形成。
3. **"Role of Pkn3 in Cardiac Hypertrophy: Evidence from Knockout Mice and Antibody-based Detection"**
*作者：Wang Y, et al.*
摘要：通过比较野生型和Pkn3敲除小鼠的心脏组织，结合N端抗体验证，研究证明Pkn3通过磷酸化GSK-3β抑制病理性心肌肥厚，为心脏疾病治疗提供新靶点。
4. **"Development and Validation of a Monoclonal Antibody Targeting the N-terminal Domain of Murine Pkn3 for Flow Cytometry"**
*作者：Garcia R, et al.*
摘要：报道了一种针对小鼠Pkn3 N端的单克隆抗体的开发，优化了其在流式细胞术中的应用，并证实其在免疫细胞亚群中检测Pkn3表达的可靠性。
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**提示**：实际文献需通过PubMed、Google Scholar或抗体供应商官网(如Abcam、CST)搜索关键词“Mouse Pkn3 antibody N-terminal”获取。部分抗体可能仅存在于商业技术文档中，未发表为研究论文。
The Mouse Pkn3 (N-term) antibody is a polyclonal or monoclonal antibody specifically designed to detect the N-terminal region of Protein Kinase N3 (PKN3) in mouse-derived samples. PKN3. also known as PRKCL3 or PKNβ, is a serine/threonine kinase belonging to the protein kinase C (PKC) superfamily. It shares structural homology with other PKN family members (PKN1 and PKN2), featuring an N-terminal regulatory domain with a PB1 (Phox and Bem1) motif and a C-terminal catalytic kinase domain. PKN3 plays roles in cell signaling pathways regulating cytoskeletal dynamics, cell migration, and survival, with implications in cancer progression and angiogenesis.
The N-terminal region targeted by this antibody is critical for protein-protein interactions, particularly through its PB1 domain, which facilitates binding to other PB1-containing proteins involved in signaling cascades. Researchers use this antibody in applications such as Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to study PKN3 expression, localization, and interaction partners in mouse tissues or cell lines. Its specificity is often validated via knockout/knockdown controls or peptide blocking assays.
PKN3 is of interest due to its overexpression in certain cancers, including prostate and breast tumors, and its potential as a therapeutic target. The Mouse Pkn3 (N-term) antibody thus serves as a key tool for elucidating PKN3's physiological and pathological functions in preclinical models.
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