Rabbit Polyclonal AFF1 Antibody

The AFF1 antibody targets the AFF1 protein (AF4/FMR2 family member 1), a key component of the super elongation complex (SEC) involved in transcriptional regulation. AFF1. also known as AF4. plays a critical role in RNA polymerase II-mediated transcriptional elongation, particularly during development and hematopoiesis. It interacts with other SEC members, such as ENL and ELL, to facilitate chromatin remodeling and promote gene expression by releasing paused polymerases. AFF1 is notably implicated in acute leukemias, especially in mixed-lineage leukemia (MLL)-rearranged cases, where chromosomal translocations create oncogenic MLL-AFF1 fusion proteins. These fusions aberrantly recruit SEC components, driving uncontrolled expression of pro-leukemic genes like HOXA9 and MEIS1. Antibodies against AFF1 are essential tools for studying its function, localization, and interactions in both normal and pathological contexts. They are widely used in techniques like Western blotting, immunoprecipitation, and immunofluorescence to investigate transcriptional mechanisms, leukemia biology, and potential therapeutic targets. Validated AFF1 antibodies help elucidate its role in developmental disorders and cancers, offering insights into disease pathways and treatment strategies.