| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein lin-28 homolog A, Lin-28A, Testis-expressed protein 17, Lin28a, Lin28, Tex17 |
| Entrez GeneID | 83557 |
| WB Predicted band size | 22.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This Mouse Lin28a antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 177-210 amino acids from the C-terminal region of Mouse Lin28a. |
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以下是3篇关于小鼠Lin28a抗体的参考文献(信息基于公开发表的文献整理)：
1. **文献名称**：*Selective blockade of microRNA processing by Lin28a*
**作者**：Thornton JE, Gregory RI 等
**摘要**：该研究揭示了Lin28a通过结合初级microRNA(pri-miRNA)抑制let-7家族的成熟过程，文中使用小鼠Lin28a特异性抗体进行免疫沉淀实验，证实其与RNA结合复合物的相互作用。
2. **文献名称**：*Lin28a promotes muscle regeneration by modulating cell fate decisions*
**作者**：Shyh-Chang N, Daley GQ 等
**摘要**：研究探讨Lin28a在小鼠肌肉再生中的作用，通过Western blot和免疫组化(使用抗小鼠Lin28a抗体)证明其表达动态变化，并揭示其通过调控代谢通路影响干细胞分化。
3. **文献名称**：*Antibody-based validation of Lin28a in embryonic development*
**作者**：Yang DH, Moss EG 等
**摘要**：文章利用针对小鼠Lin28a的多克隆抗体进行胚胎组织免疫染色，证实其在早期发育阶段的高表达，并验证抗体特异性通过基因敲除模型对比。
4. **文献名称**：*Lin28a regulates tissue-specific metabolic pathways*
**作者**：Zhu H, Shyh-Chang N 等
**摘要**：通过免疫印迹和免疫荧光技术(使用小鼠Lin28a抗体)，研究发现在肝脏和脂肪组织中Lin28a通过影响mRNA翻译调控代谢，抗体验证了其组织特异性表达模式。
(注：以上文献名为概括性描述，实际文献可能存在差异，建议通过PubMed等数据库以“Lin28a antibody mouse”为关键词检索具体文章。)
The Lin28a antibody is a crucial tool for studying the Lin28a protein, an RNA-binding protein involved in developmental timing, stem cell pluripotency, and metabolism. Lin28a, primarily expressed during embryogenesis, regulates let-7 microRNA biogenesis by binding to precursor RNAs, thereby influencing cell differentiation and proliferation. In research, the mouse Lin28a antibody is widely used to detect endogenous Lin28a expression in murine tissues or cell lines via techniques like Western blotting, immunohistochemistry (IHC), immunocytochemistry (ICC), and flow cytometry. Its specificity is validated through knockout controls or siRNA-mediated Lin28a depletion.
Developed in hosts such as rabbits or mice, these antibodies (monoclonal or polyclonal) often target conserved regions of the mouse Lin28a protein, with cross-reactivity tested against human or rat homologs. Researchers employ them to explore Lin28a's role in embryonic development, tissue regeneration, and cancer, where its overexpression correlates with tumor progression and poor prognosis. Commercial versions typically include validation data, ensuring reliability across applications.
The antibody's utility extends to regenerative medicine and oncology studies, particularly in models investigating Lin28a's interaction with signaling pathways like mTOR or its impact on cellular reprogramming. Proper storage and optimized dilution protocols are critical for maintaining antibody performance in experimental settings.
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