| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Ras-related protein Rab-11B, GTP-binding protein YPT3, RAB11B, YPT3 |
| Entrez GeneID | 9230 |
| WB Predicted band size | 24.5kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Rat |
| Immunogen | This RAB11B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 164-193 amino acids from the C-terminal region of human RAB11B. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是3条关于RAB11B抗体的参考文献(虚拟示例，仅供参考)：
1. **"RAB11B regulates vesicular trafficking in neuronal dendrites"**
- 作者：Chen L, et al.
- 摘要：研究利用RAB11B抗体通过免疫荧光和Western blot技术，揭示了RAB11B蛋白在神经元树突囊泡运输中的关键作用，并发现其缺失会导致突触蛋白递送异常。
2. **"Role of RAB11B in cancer cell migration and invasion"**
- 作者：Smith JR, et al.
- 摘要：通过RAB11B抗体介导的免疫组化和基因沉默实验，证明RAB11B通过调控整合素回收途径促进乳腺癌细胞的迁移和侵袭能力。
3. **"RAB11B antibody validation for subcellular localization studies"**
- 作者：Wang H, et al.
- 摘要：文章系统验证了多种RAB11B抗体的特异性，确认其在共聚焦显微镜下标记回收内体(recycling endosomes)的可靠性，为后续功能研究提供工具支持。
4. **"RAB11B modulates insulin secretion in pancreatic β-cells"**
- 作者：Garcia M, et al.
- 摘要：使用RAB11B抗体结合免疫沉淀技术，发现RAB11B通过与SNARE蛋白互作调控胰岛素分泌囊泡的胞吐过程，影响血糖稳态。
(注：以上文献为假设性示例，实际文献需通过PubMed/Google Scholar等平台检索关键词“RAB11B antibody”或“RAB11B function”。)
**Background of RAB11B Antibody**
RAB11B is a member of the RAB family of small GTPases, which regulate vesicular trafficking and membrane dynamics in eukaryotic cells. Specifically, RAB11B localizes to recycling endosomes, the trans-Golgi network, and secretory vesicles, playing critical roles in cargo sorting, cytokinesis, ciliogenesis, and autophagy. It is closely related to RAB11A but exhibits distinct tissue expression patterns and functional specializations, particularly in neuronal and secretory tissues.
Antibodies against RAB11B are essential tools for studying its spatial-temporal distribution, interactions, and roles in cellular processes. These antibodies are typically validated for applications such as Western blotting, immunofluorescence, and immunoprecipitation to detect endogenous RAB11B protein levels or track its subcellular localization. Dysregulation of RAB11B has been implicated in pathological conditions, including cancer metastasis, neurodegenerative diseases (e.g., Alzheimer’s), and ciliopathies, underscoring its biological and clinical relevance.
High-quality RAB11B antibodies are characterized by specificity, sensitivity, and minimal cross-reactivity with homologous proteins like RAB11A. Researchers often employ knockdown/knockout validation or isoform-specific controls to confirm antibody reliability. Commercial RAB11B antibodies are available from multiple suppliers, with host species (e.g., rabbit, mouse) and clonality (monoclonal/polyclonal) options tailored to experimental needs.
In summary, RAB11B antibodies are indispensable for elucidating the molecular mechanisms of intracellular trafficking and disease pathogenesis linked to RAB11B dysfunction.
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