Mouse Monoclonal EEF1E1 Antibody

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     All lanes : Anti-EEF1E1 Antibody at 1:2000-1:4000 dilution Lane 1: HepG2 whole cell lysates Lane 2: human testis lysates Lane 3: A549 whole cell lysates Lane 4: A431 whole cell lysates Lane 5: Jurkat whole cell lysates Lane 6: mouse brain lysates                                                                                                                                         Lysates/proteins at 20 μg per lane. Secondary Goat Anti-mouse IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 20 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human cervical epithelial adenocarcinoma cell line) cells labeling EEF1E1 with P33132 at 1/25 dilution, followed by Dylight® 488-conjugated goat anti-mouse IgG   secondary antibody at 1/200 dilution (green). Immunofluorescence image showing cytoplasm staining on HeLa cell line. Cytoplasmic actin is detected with Dylight® 554 Phalloidin  at 1/100 dilution (red).The nuclear counter stain is DAPI (blue).    

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     Overlay histogram showing Hela cells stained with P33132 (green line).  The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min.  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33132,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821)) at 1/400 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was mouse IgG2b (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

The eukaryotic translation elongation factor 1 epsilon-1 (EEF1E1), also known as eEF1Bε or eEF1δ, is a subunit of the eEF1B complex involved in protein synthesis. This complex facilitates the exchange of GDP for GTP on eEF1A, enabling the recycling of eEF1A for subsequent rounds of elongation during mRNA translation. Beyond its canonical role in translation, EEF1E1 has been implicated in diverse cellular processes, including transcriptional regulation, apoptosis, and interactions with viral proteins. Dysregulation of EEF1E1 has been observed in cancers, such as hepatocellular carcinoma and breast cancer, where it may influence tumor progression, metastasis, or chemoresistance. It has also been linked to oxidative stress responses and neurodegenerative diseases.

EEF1E1 antibodies are essential tools for studying its expression, localization, and function. These antibodies are widely used in techniques like Western blotting, immunohistochemistry (IHC), and immunofluorescence (IF) to investigate EEF1E1’s tissue-specific distribution, subcellular localization (primarily cytoplasmic), and alterations under pathological conditions. Validated antibodies help identify EEF1E1 as a potential biomarker or therapeutic target. Recent studies explore its interactions with non-coding RNAs or viral components, expanding its role in infection biology. Commercial EEF1E1 antibodies are typically raised in rabbits or mice, with specificity confirmed via knockout controls. Researchers prioritize antibodies with high affinity and minimal cross-reactivity to ensure reliability in experimental models.