| WB | 1/1000-1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | DNA (cytosine-5)-methyltransferase 3B, Dnmt3b, DNA methyltransferase HsaIIIB, DNA MTase HsaIIIB, MHsaIIIB, DNMT3B |
| Entrez GeneID | 1789 |
| WB Predicted band size | 95.8kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This Dnmt3b antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 389-417 amino acids from human Dnmt3b. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
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以下是关于Dnmt3b抗体的3篇参考文献，涵盖其功能研究和应用方向：
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1. **文献名称**：*DNA methyltransferases Dnmt3a and Dnmt3b are essential for de novo methylation and mammalian development*
**作者**：Okano, M., et al.
**摘要**：本研究通过基因敲除小鼠模型，发现Dnmt3b缺失导致胚胎致死及基因组甲基化异常，利用特异性抗体证实其在胚胎组织和器官发生中的高表达，揭示了Dnmt3b对哺乳动物发育的关键作用。
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2. **文献名称**：*Overexpression of Dnmt3b in human colorectal cancer through epigenetic alteration*
**作者**：Saito, Y., et al.
**摘要**：通过免疫组化(使用Dnmt3b抗体)和甲基化分析，发现结直肠癌中Dnmt3b显著过表达，且与抑癌基因异常高甲基化及患者预后不良相关，提示其作为癌症治疗靶点的潜力。
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3. **文献名称**：*Dnmt3b is required for DNA methylation during gametogenesis and embryonic development*
**作者**：Kaneda, M., et al.
**摘要**：研究利用Dnmt3b抗体检测生殖细胞和早期胚胎中的蛋白定位，发现Dnmt3b特异性介导印记基因和转座元件的甲基化，其功能缺失导致基因组印记紊乱及发育缺陷。
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4. **文献名称**：*Dnmt3b interacts with the LSD1/KDM1A histone demethylase to repress cryptic promoters*
**作者**：Wang, J., et al.
**摘要**：通过免疫共沉淀(使用Dnmt3b抗体)和ChIP-seq分析，揭示Dnmt3b与组蛋白去甲基化酶LSD1协同抑制基因组中异常转录起始位点，维持表观遗传稳定性。
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这些文献分别从发育生物学、癌症表观遗传、生殖细胞调控及表观复合物互作等角度，展示了Dnmt3b抗体的实验应用(如WB、IHC、Co-IP等)及其功能研究。
The Dnmt3b antibody is a crucial tool in epigenetic research, specifically targeting the DNA methyltransferase 3B (DNMT3B) enzyme, a key player in establishing de novo DNA methylation patterns during development. DNMT3B, along with DNMT3A, catalyzes the transfer of methyl groups to cytosine residues in CpG dinucleotides, shaping gene expression profiles and maintaining genomic stability. Unlike DNMT1. which preserves methylation during replication, DNMT3B is essential for embryonic development, germ cell differentiation, and tissue-specific methylation.
Antibodies against DNMT3B enable researchers to study its expression, localization, and functional interactions in diverse biological contexts. They are widely used in techniques like Western blotting, immunohistochemistry, and chromatin immunoprecipitation (ChIP) to investigate DNMT3B's role in cellular processes, including X-chromosome inactivation, imprinting, and heterochromatin formation. Dysregulation of DNMT3B is linked to diseases such as cancer, immunodeficiency syndromes (e.g., ICF syndrome), and developmental disorders, making these antibodies vital for mechanistic and diagnostic studies.
Commercial DNMT3B antibodies are typically validated for specificity using knockout cell lines or peptide competition assays. However, variability in epitope recognition and cross-reactivity with homologous proteins (e.g., DNMT3A) necessitates careful experimental optimization. Researchers rely on these reagents to explore DNMT3B's involvement in epigenetic reprogramming, aging, and therapeutic targeting of methylation-related pathologies.
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