Rabbit Polyclonal PLOD1(N-term) Antibody

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     All lanes : Anti-PLOD1 Antibody (N-term) at 1:2000 dilution Lane 1: A431 whole cell lysates Lane 2: MCF-7 whole cell lysates Lane 3: U-2OS whole cell lysates Lane 4: U-87 MG whole cell lysates Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG,  (H+L), Peroxidase conjugated at 1/10000 dilution Predicted band size : 84 kDa Blocking/Dilution buffer: 5% NFDM/TBST.    

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     Overlay histogram showing U-87 MG cells stained with P33134 (green line).  The cells were fixed with 2% paraformaldehyde (10 min).  The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (P33134,  1:25 dilution) for 60 min at 37ºC.  The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed(NA168821) at 1/400 dilution for 40 min at 37ºC.  Isotype control antibody (blue line) was rabbit IgG (1μg/1x10^6 cells) used under the same conditions.  Acquisition of >10, 000 events was performed.    

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     P33134 staining PLOD1 in Human heart tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed,  paraffin-embedded sections).  Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6).  Samples were incubated with primary antibody (1/25) for 1 hours at 37°C.  A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.    

The PLOD1 (N-term) antibody is designed to target the N-terminal region of procollagen-lysine,2-oxoglutarate 5-dioxygenase 1 (PLOD1), a key enzyme in collagen biosynthesis. PLOD1. also known as lysyl hydroxylase 1 (LH1), catalyzes the hydroxylation of lysine residues in collagen precursors, a critical step for stabilizing intermolecular cross-links in mature collagen fibrils. Mutations in the PLOD1 gene are linked to Ehlers-Danlos syndrome type VI (EDS-VI), a rare autosomal recessive disorder characterized by skin fragility, joint hypermobility, and ocular abnormalities.

The PLOD1 (N-term) antibody is widely used in research to study collagen metabolism disorders, particularly in models of EDS-VI or connective tissue pathologies. It enables detection of PLOD1 expression levels via techniques like Western blotting, immunohistochemistry, and immunofluorescence, aiding in the evaluation of protein localization and abundance in tissues or cell lines. Specificity for the N-terminal region helps distinguish PLOD1 from other lysyl hydroxylase isoforms (e.g., PLOD2/3). Validations often include knockdown controls or PLOD1-deficient samples to confirm target specificity.

This antibody also serves as a tool to explore PLOD1's roles in extracellular matrix homeostasis, wound healing, and diseases like fibrosis or cancer metastasis, where collagen remodeling is dysregulated. Its application contributes to both diagnostic research and therapeutic development for connective tissue disorders.