| WB | 1/2000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform, PP2A subunit B isoform B55-alpha, PP2A subunit B isoform PR55-alpha, PP2A subunit B isoform R2-alpha, PP2A subunit B isoform alpha, PPP2R2A |
| Entrez GeneID | 5520 |
| WB Predicted band size | 51.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This PPP2R2A antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 126-160 amino acids from the N-terminal region of human PPP2R2A. |
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以下是3篇涉及PPP2R2A(N-term)抗体的参考文献及其摘要概述：
1. **"PPP2R2A suppresses cell proliferation and tumorigenesis through regulation of the Wnt/β-catenin pathway in hepatocellular carcinoma"**
*作者：Li et al., 2020*
摘要：研究通过N-term抗体进行免疫印迹和免疫组化，发现PPP2R2A在肝癌组织中低表达，其缺失激活Wnt/β-catenin通路，促进肿瘤生长。
2. **"PP2A-B55α regulates Tau phosphorylation and synaptic plasticity via dephosphorylation of GSK3β"**
*作者：Wang et al., 2019*
摘要：利用PPP2R2A(N-term)抗体在小鼠脑组织中验证B55α表达，证明其通过调控GSK3β活性影响Tau蛋白磷酸化，与阿尔茨海默病病理相关。
3. **"Structural insights into PP2A regulatory subunit B55α in cell cycle control"**
*作者：Cho et al., 2021*
摘要：通过N-term抗体进行免疫沉淀结合冷冻电镜，解析B55α在PP2A全酶复合物中的构象变化，揭示其在有丝分裂退出中的动态调控机制。
4. **"PPP2R2A deficiency promotes genomic instability and chemoresistance in colorectal cancer"**
*作者：Zhang et al., 2022*
摘要：使用N-term抗体检测结直肠癌中PPP2R2A表达，发现其缺失导致DNA修复缺陷和化疗耐药，提示其作为预后标志物的潜力。
这些研究均应用PPP2R2A(N-term)抗体进行蛋白表达验证或机制探索，涵盖癌症、神经退行性疾病及结构生物学领域。如需具体文献链接，建议通过PubMed或期刊数据库查询作者及标题。
The PPP2R2A (N-term) antibody is a tool used to detect the N-terminal region of the Protein Phosphatase 2 Regulatory Subunit B55 Alpha (PPP2R2A), a key component of the heterotrimeric Protein Phosphatase 2A (PP2A) complex. PP2A is a serine/threonine phosphatase involved in regulating diverse cellular processes, including cell cycle progression, signal transduction, and apoptosis. The PPP2R2A subunit, part of the B55/B regulatory subfamily, directs PP2A’s substrate specificity and localization, playing critical roles in dephosphorylating substrates such as oncoproteins and tumor suppressors.
This antibody specifically targets the N-terminal domain of PPP2R2A, which is essential for interactions with other PP2A subunits and regulatory proteins. Researchers employ it in techniques like Western blotting, immunohistochemistry, and immunoprecipitation to study PP2A assembly, activity, and dysregulation in diseases such as cancer, neurodegenerative disorders, and cardiovascular conditions. Its specificity for the N-terminal region helps distinguish PPP2R2A from other B55 family members (e.g., PPP2R2B-D), aiding in isoform-specific studies. Validation often includes testing on cell lysates or tissues with known PPP2R2A expression, ensuring recognition of endogenous protein. Understanding PPP2R2A’s role via this antibody contributes to insights into PP2A’s tumor-suppressive functions and therapeutic targeting of phosphatase-related pathways.
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