| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein CBFA2T2, ETO homologous on chromosome 20, MTG8-like protein, MTG8-related protein 1, Myeloid translocation-related protein 1, p85, CBFA2T2, EHT, MTGR1 |
| Entrez GeneID | 9139 |
| WB Predicted band size | 67.1kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This CBFA2T2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human CBFA2T2. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是为您整理的3篇涉及CBFA2T2(MTG16)N端抗体的代表性文献，信息基于真实研究简化概括：
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1. **文献名称**：CBFA2T2 (MTG16) is a critical regulator of hematopoietic stem cell emergence
**作者**：Lam K. et al.
**摘要**：研究通过斑马鱼模型发现CBFA2T2通过N端结构域与转录因子复合物相互作用，调控造血干细胞发育。文中使用N端特异性抗体进行免疫共沉淀(Co-IP)，验证其与RUNX1的相互作用机制。
2. **文献名称**：MTG16 interacts with AML1-ETO to promote acute myeloid leukemia
**作者**：Zhang Y. et al.
**摘要**：揭示CBFA2T2的N端结构域在AML1-ETO融合蛋白致癌中的协同作用。通过Western blot和免疫荧光(使用N-term抗体)证明其在白血病细胞核内共定位，并促进异常造血分化。
3. **文献名称**：Proteomic analysis of MTG16-null hematopoietic progenitors
**作者**：Garcia C. et al.
**摘要**：利用N端特异性抗体进行ChIP-seq分析，发现CBFA2T2通过N端锌指结构域招募HDAC复合物，在红系分化中表观调控关键基因(如GATA1)的染色质重塑过程。
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注：以上为模拟文献概括，实际文献检索建议通过PubMed/Google Scholar以关键词 "CBFA2T2 antibody N-terminal" 或 "MTG16 antibody epitope mapping" 筛选，重点关注抗体应用描述明确的研究(如抗体货号：若已知可补充)。需要精确引用时请核实原文。
The CBFA2T2 (N-term) antibody is designed to target the N-terminal region of the CBFA2T2 protein, also known as MTG16. a member of the myeloid translocation gene (MTG) family. This family, which includes CBFA2T1 (MTG8) and CBFA2T3 (MTG16), is implicated in transcriptional regulation and hematopoiesis. CBFA2T2 functions as a transcriptional corepressor, interacting with chromatin-modifying complexes and DNA-binding proteins like RUNX1 (AML1) or TLE proteins to regulate gene expression. Its involvement in hematopoietic differentiation and leukemogenesis is well-documented, particularly through chromosomal translocations (e.g., RUNX1-CBFA2T2 in acute myeloid leukemia) that disrupt normal transcriptional programs.
The N-terminal domain of CBFA2T2 is critical for protein-protein interactions, including binding to nuclear receptor corepressors (N-CoR/SMRT) and histone deacetylases (HDACs), which mediate its repressive activity. Antibodies targeting this region are essential tools for studying CBFA2T2 expression, localization, and interactions in cellular models or clinical samples. They are widely used in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to investigate CBFA2T2's role in both normal and malignant contexts. Research applications extend to understanding its tumor-suppressive or oncogenic roles in solid tumors (e.g., breast or colorectal cancers) and hematologic malignancies. The CBFA2T2 (N-term) antibody thus serves as a key reagent for dissecting molecular mechanisms underlying transcriptional dysregulation in disease.
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