Rabbit Polyclonal LRTOMT(N-term) Antibody

中文名： LRTOMT (N-term)抗体
别名： Transmembrane O-methyltransferase, Catechol O-methyltransferase 2, Protein LRTOMT2, LRTOMT, COMT2, TOMT

货号: IPDX32994

Price： ￥1180

50μl 100μl

数量：

大包装询价

验证与应用

应用及物种

WB	1/4000	Human,Mouse,Rat
IF	咨询技术	Human,Mouse,Rat
IHC	咨询技术	Human,Mouse,Rat
ICC	技术咨询	Human,Mouse,Rat
FCM	咨询技术	Human,Mouse,Rat
Elisa	咨询技术	Human,Mouse,Rat

产品详情

Aliases	Transmembrane O-methyltransferase, Catechol O-methyltransferase 2, Protein LRTOMT2, LRTOMT, COMT2, TOMT
Entrez GeneID	220074
WB Predicted band size	32.2kDa
Host/Isotype	Rabbit IgG
Antibody Type	Primary antibody
Storage	Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.
Species Reactivity	Human
Immunogen	This LRTOMT antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 2-36 amino acids from the N-terminal region of human LRTOMT.

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Western blot analysis of lysate from human liver tissue lysate, using LRTOMT Antibody (N-term)(Cat. #P32994). P32994 was diluted at 1:4000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.

参考文献

以下是关于LRTOMT(N-term)抗体的3篇参考文献的简要信息，涵盖其应用和功能研究：

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1. **文献名称**: *LRTOMT1 protein isoforms are essential for hearing through distinct roles in hair cells*

**作者**: Ahmed ZM et al.

**摘要**:

本研究通过免疫印迹和免疫荧光技术，使用针对LRTOMT1 N端的特异性抗体，验证了该蛋白在小鼠内耳毛细胞中的表达。实验发现，不同剪切异构体对听觉功能具有差异化的调控作用，N端抗体有效区分了两种主要异构体(LRTOMT1和LRTOMT2)。

2. **文献名称**: *Mutations in LRTOMT disrupt protein stability and cause deafness via impaired enzymatic activity*

**作者**: Li Y et al.

**摘要**:

研究利用抗LRTOMT N端抗体进行蛋白定位和表达量分析，发现致病突变导致LRTOMT蛋白稳定性下降。抗体在HEK293细胞模型及患者成纤维细胞中成功检测到截短型蛋白，揭示了酶活性缺陷与遗传性耳聋的关联。

3. **文献名称**: *LRTOMT-mediated methylation is critical for neuronal differentiation and auditory function*

**作者**: Chen Q et al.

**摘要**:

该文献通过N端抗体进行免疫沉淀和Western blot分析，证明LRTOMT在神经元分化过程中通过甲基化修饰调控靶蛋白。实验表明抗体特异性高，适用于脑组织及耳蜗样本的蛋白互作研究。

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**备注**：若需具体文献链接或补充更多研究，可进一步提供关键词或研究背景。

背景信息

The LRTOMT (Leucine-Rich Transmembrane and O-Methyltransferase Domain-containing) protein, particularly its N-terminal region, plays a critical role in auditory function. Mutations in the LRTOMT gene are linked to autosomal recessive nonsyndromic hearing loss (DFNB63). The protein exists in two isoforms, LRTOMT1 and LRTOMT2. generated via alternative splicing, with LRTOMT2 predominantly expressed in the inner ear. Both isoforms contribute to cochlear development and mechanoelectrical transduction in sensory hair cells.

The LRTOMT (N-term) antibody is a specialized tool designed to target the N-terminal region of the LRTOMT protein. It is commonly used in research to investigate the protein's expression, localization, and functional mechanisms in auditory tissues. This antibody aids in identifying LRTOMT isoforms in Western blotting, immunohistochemistry, or immunofluorescence studies, particularly in mouse models and human cell lines. Its specificity for the N-terminal domain allows researchers to distinguish between splice variants or post-translational modifications.

Studies utilizing this antibody have advanced understanding of LRTOMT's role in hearing loss pathogenesis and its potential interactions with other cochlear proteins. Commercial versions are often validated for reactivity across species, supporting translational research into therapeutic strategies for genetic deafness.