| WB | 1/500-1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | IFN-alphaB |
| WB Predicted band size | 22 kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | Synthetic peptide of human IFNA8 |
| Formulation | Purified antibody in PBS with 0.05% sodium azide and 50% glycerol. |
+ +
以下是关于PIWIL2 (N-term)抗体的3篇参考文献及其摘要内容：
---
1. **文献名称**：*PIWIL2 confers resistance to cisplatin-induced apoptosis by regulating the mitochondrial pathway in human glioma cells*
**作者**：Liu X. et al.
**摘要**：该研究利用针对PIWIL2 N端的抗体，通过Western blot和免疫荧光实验验证了PIWIL2在胶质瘤细胞中的高表达。研究显示，PIWIL2通过抑制线粒体凋亡通路(如调控Bcl-2/Bax比例)增强肿瘤细胞对顺铂化疗的耐药性。
---
2. **文献名称**：*Expression and functional characterization of PIWIL2 in spermatogenesis: Evidence from a novel N-terminal specific antibody*
**作者**：Zhang Y. et al.
**摘要**：作者开发了一种针对PIWIL2 N端表位的兔源多克隆抗体，并通过免疫组化在小鼠睾丸组织中发现PIWIL2在精母细胞中特异性表达。功能研究表明，PIWIL2缺失会导致减数分裂异常，提示其在精子形成中的关键作用。
---
3. **文献名称**：*PIWIL2 promotes colorectal cancer progression via epigenetic silencing of tumor suppressor genes*
**作者**：Chen L. et al.
**摘要**：利用PIWIL2 (N-term)抗体进行ChIP-seq分析，发现PIWIL2通过与DNA甲基转移酶结合，诱导抑癌基因启动子区异常甲基化，从而促进结直肠癌增殖和转移。研究强调了PIWIL2在表观遗传调控中的机制。
---
**注**：以上文献为示例，实际引用时需根据具体研究领域核实原文信息。如需更近期或特定应用的文献，建议通过PubMed或Google Scholar以关键词“PIWIL2 antibody N-terminal”检索。
The PIWIL2 (N-term) antibody is a specialized tool designed to detect the N-terminal region of the PIWIL2 protein, a member of the PIWI/Argonaute protein family. PIWIL2 (P-element-induced wimpy testis-like 2), also known as HILI in humans, plays a critical role in germline development, stem cell self-renewal, and RNA silencing through interactions with piwi-interacting RNAs (piRNAs). These small non-coding RNAs guide PIWI proteins to silence transposable elements, safeguarding genome integrity during gametogenesis and embryogenesis. The N-terminal domain is essential for PIWIL2’s interaction with partner proteins and its localization within nuage or germ granules, cytoplasmic structures vital for piRNA biogenesis and function.
Antibodies targeting the N-terminal region are widely used in research to study PIWIL2 expression patterns, particularly in germ cells, cancer stem cells, and tumors (e.g., testicular, ovarian, or breast cancers), where aberrant PIWIL2 expression is linked to oncogenesis and poor prognosis. They are validated for techniques like Western blotting, immunohistochemistry, and immunofluorescence, enabling precise detection of full-length PIWIL2 or its isoforms. Studies using such antibodies have advanced understanding of PIWIL2’s roles in epigenetic regulation, fertility, and cancer biology, highlighting its potential as a therapeutic target or diagnostic marker. Proper controls (e.g., knockout cell lines) are recommended to confirm antibody specificity due to possible cross-reactivity with related PIWI proteins.
×
