| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Protein SDA1 homolog, Nucleolar protein 130, SDA1 domain-containing protein 1, hSDA, SDAD1, NUC130 |
| Entrez GeneID | 55153 |
| WB Predicted band size | 79.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This SDAD1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 596-629 amino acids from the C-terminal region of human SDAD1. |
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以下是关于SDAD1抗体的3篇参考文献的简要总结(文献信息为虚构示例，仅用于演示格式)：
1. **文献名称**: *SDAD1 regulates viral RNA synthesis by interacting with NS5B in hepatitis C virus replication*
**作者**: Zhang L, et al.
**摘要**: 本研究利用SDAD1特异性抗体进行免疫共沉淀实验，揭示了SDAD1通过与HCV病毒蛋白NS5B相互作用，促进病毒RNA的合成。抗体验证了SDAD1在感染细胞中的定位及表达水平。
2. **文献名称**: *The role of SDAD1 in colorectal cancer progression via Wnt/β-catenin signaling*
**作者**: Wang Y, et al.
**摘要**: 通过免疫组化(IHC)和Western blot使用SDAD1抗体，发现SDAD1在结直肠癌中高表达，并通过激活Wnt通路促进肿瘤侵袭。抗体特异性通过siRNA敲除实验验证。
3. **文献名称**: *SDAD1 is essential for innate immune response against RNA viruses*
**作者**: Chen R, et al.
**摘要**: 研究利用SDAD1抗体进行免疫荧光染色，证实SDAD1定位于细胞质RNA颗粒中，并参与RIG-I介导的抗病毒信号通路。敲低SDAD1显著抑制干扰素产生。
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**提示**：实际文献需通过PubMed/Google Scholar检索关键词(如“SDAD1 antibody”、“SDAD1 function”)，并筛选涉及抗体应用的实验研究(如Western blot、IP、IHC等)。建议结合SDAD1的别名(如C14orf166)扩大检索范围。
The SDAD1 (SDA1 domain-containing protein 1) antibody is a research tool targeting the SDAD1 protein, a conserved eukaryotic protein implicated in transcriptional regulation and RNA metabolism. SDAD1. also known as NEFA-interacting nuclear protein (NIP30), contains a structured SDA1 domain critical for its interactions with components of the transcriptional machinery, including RNA polymerase II and chromatin-modifying complexes. Studies suggest SDAD1 plays roles in mRNA processing, chromatin remodeling, and antiviral innate immunity by regulating the expression of interferon-stimulated genes.
SDAD1 antibodies are typically developed in hosts like rabbits or mice using immunogenic peptide sequences or recombinant protein fragments. These antibodies are widely utilized in techniques such as Western blotting, immunofluorescence, and immunoprecipitation to study SDAD1's subcellular localization (primarily nuclear), expression patterns across tissues, and dynamic interactions under physiological or stress conditions.
Research highlights SDAD1's involvement in diseases, including viral infections and cancers. For instance, SDAD1 deficiency has been linked to dysregulated immune responses, while its overexpression in certain tumors correlates with poor prognosis. The antibody serves as a critical reagent for dissecting SDAD1's molecular functions and its potential as a therapeutic target. Validation of SDAD1 antibodies often includes knockout cell line controls to ensure specificity.
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