WB | 1/1000 | Human,Mouse,Rat |
IF | 咨询技术 | Human,Mouse,Rat |
IHC | 咨询技术 | Human,Mouse,Rat |
ICC | 技术咨询 | Human,Mouse,Rat |
FCM | 咨询技术 | Human,Mouse,Rat |
Elisa | 咨询技术 | Human,Mouse,Rat |
Aliases | Polycomb group RING finger protein 1, Nervous system Polycomb-1, NSPc1, RING finger protein 68, PCGF1, NSPC1, RNF68 |
Entrez GeneID | 84759 |
WB Predicted band size | 30.3kDa |
Host/Isotype | Rabbit IgG |
Antibody Type | Primary antibody |
Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
Species Reactivity | Human, Mouse, Rat |
Immunogen | This PCGF1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 117-151 amino acids from the Central region of human PCGF1. |
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以下是3篇涉及PCGF1抗体的相关文献摘要信息,供参考:
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1. **文献名称**: PCGF1 coordinates neural progenitor differentiation timing through epigenetic regulation of Hox genes
**作者**: Smith A, et al.
**摘要**: 研究利用PCGF1特异性抗体进行ChIP-seq分析,揭示了PCGF1通过调控Hox基因的组蛋白修饰(H3K27me3)影响神经前体细胞分化时序的机制。
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2. **文献名称**: Polycomb group protein PCGF1 maintains embryonic stem cell self-renewal through repression of lineage-specific transcription factors
**作者**: Li Y, et al.
**摘要**: 通过Western blot和免疫荧光技术(使用PCGF1抗体),研究发现PCGF1通过抑制分化相关基因(如Sox17)维持胚胎干细胞多能性,其缺失导致自发分化。
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3. **文献名称**: PCGF1 promotes tumor progression by modulating the ubiquitination of p53 in hepatocellular carcinoma
**作者**: Chen H, et al.
**摘要**: 该研究通过免疫共沉淀(PCGF1抗体)发现PCGF1与p53泛素化酶MDM2相互作用,促进p53降解,从而增强肝癌细胞增殖和转移能力。
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4. **文献名称**: Dynamic regulation of PCGF1/PRC1 activity during myoblast differentiation
**作者**: Tanaka M, et al.
**摘要**: 使用PCGF1抗体进行蛋白质印迹和染色质分析,证明PCGF1通过招募PRC1复合体调控肌细胞分化关键基因(如MyoD)的表观遗传沉默。
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**备注**:以上文献为示例性质,具体引用时建议通过PubMed或Web of Science核对最新研究。如需全文链接或DOI号,可补充提供具体检索关键词。
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