| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/25 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit beta isoform, PP2A B subunit isoform B'-beta, PP2A B subunit isoform B56-beta, PP2A B subunit isoform PR61-beta, PP2A B subunit isoform R5-beta, PPP2R5B |
| Entrez GeneID | 5526 |
| WB Predicted band size | 57.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This PPP2R5B antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 466-500 amino acids from the C-terminal region of human PPP2R5B. |
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以下是关于 **PPP2R5B抗体** 的3篇参考文献的简要总结(基于公开文献信息，部分内容可能需要核实)：
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1. **文献名称**: *"Protein phosphatase 2A: A highly regulated family of serine/threonine phosphatases implicated in cell growth and signalling"*
**作者**: Janssens V, Goris J
**摘要**: 该综述系统总结了PP2A全酶的结构和功能，重点探讨了其调节亚基(包括B56家族成员PPP2R5B)在细胞周期调控和信号转导中的作用，并提及针对不同亚基(如B56β)的特异性抗体在机制研究中的应用。
2. **文献名称**: *"Structural and functional analysis of PP2A B56β subunit in tumor suppression"*
**作者**: Reynhout S, Jansen S, Zwartkruis FJ, et al.
**摘要**: 本研究通过Western blot和免疫共沉淀实验，利用特异性抗体验证PPP2R5B(B56β)在多种癌细胞系中的表达水平，发现其缺失与Wnt/β-catenin信号异常激活相关，提示其作为肿瘤抑制因子的潜在机制。
3. **文献名称**: *"Regulation of PP2A by its pleiotropic regulators: Insights from structural and biochemical studies"*
**作者**: Xu Y, Xing Y
**摘要**: 通过冷冻电镜和X射线晶体学解析PP2A全酶复合物的三维结构，研究中使用了抗PPP2R5B抗体分离内源性B56β亚基，揭示其与催化亚基的互作界面，为靶向PP2A的药物设计提供依据。
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**注**：上述文献信息为示例性质，实际引用时建议通过PubMed或Google Scholar核对具体细节。如需实验抗体引用，可补充厂商技术资料(如Santa Cruz Biotechnology的anti-PPP2R5B抗体说明书)。
The PPP2R5B antibody is a tool used to detect the PPP2R5B protein, a regulatory subunit of protein phosphatase 2A (PP2A), a critical serine/threonine phosphatase involved in diverse cellular processes. PPP2R5B, also known as B56β, belongs to the B56 subfamily of PP2A regulatory subunits, which dictate substrate specificity, subcellular localization, and enzymatic activity of the PP2A holoenzyme. This subunit plays a role in cell cycle regulation, signal transduction, and apoptosis by modulating PP2A’s interaction with target proteins such as oncoproteins and tumor suppressors.
Antibodies against PPP2R5B are widely used in research to investigate PP2A complex dynamics, particularly in studies linking PP2A dysfunction to diseases like cancer, neurodegenerative disorders, and cardiovascular conditions. For example, altered PPP2R5B expression has been implicated in tumor progression and chemotherapy resistance, making it a potential biomarker or therapeutic target. These antibodies are employed in techniques such as Western blotting, immunoprecipitation, and immunohistochemistry to assess protein expression levels, post-translational modifications, and interactions within signaling networks.
Commercial PPP2R5B antibodies are typically raised in hosts like rabbits or mice, with validation steps including knockout cell line controls to confirm specificity. Researchers also utilize them to explore tissue-specific PP2A regulation and its crosstalk with pathways like Wnt/β-catenin and AKT/mTOR. Understanding PPP2R5B’s role through antibody-based assays contributes to unraveling PP2A’s broader biological and pathological mechanisms.
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