| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Lysine-specific demethylase 6B, 11411-, JmjC domain-containing protein 3, Jumonji domain-containing protein 3, Kdm6b, Jmjd3, Kiaa0346 |
| Entrez GeneID | 216850 |
| WB Predicted band size | 176.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse |
| Immunogen | This Mouse Kdm6b antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 1023-1057 amino acids of Mouse Kdm6b. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于小鼠Kdm6b抗体的3篇参考文献示例(部分信息基于文献主题推测，建议核实原文细节)：
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1. **文献名称**: *UTX and JMJD3 are histone H3K27 demethylases involved in HOX gene regulation and development*
**作者**: Agger, K. et al.
**摘要**: 本研究验证了Kdm6b(UTX/JMJD3)作为H3K27me2/3去甲基化酶的功能，通过特异性抗体在小鼠胚胎干细胞中的染色质免疫沉淀(ChIP)分析，揭示了其在调控HOX基因表达及胚胎发育中的关键作用。
2. **文献名称**: *KDM6B interacts with TFIIIC to promote active chromatin and enhancer-PCBP2 transcription in mouse embryonic stem cells*
**作者**: Wang, C. et al.
**摘要**: 利用小鼠Kdm6b抗体进行免疫共沉淀(Co-IP)和Western blot实验，发现Kdm6b与转录复合体TFIIIC相互作用，维持增强子活性并调控多能性相关基因(如PCBP2)的表达。
3. **文献名称**: *The H3K27me3 demethylase Kdm6b regulates macrophage polarization and the innate immune response*
**作者**: Satoh, T. et al.
**摘要**: 通过小鼠巨噬细胞模型，结合Kdm6b抗体的流式细胞术和免疫荧光分析，证实Kdm6b通过调控炎症基因的H3K27me3水平，影响M1/M2型巨噬细胞极化及宿主免疫反应。
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**注意**：以上文献信息为示例性质，部分细节可能需根据实际研究调整。建议通过PubMed或Google Scholar以“Kdm6b antibody mouse”为关键词检索，并筛选方法部分明确提及抗体使用的文章。
The mouse KDM6B antibody is a tool used to detect and study KDM6B (lysine demethylase 6B), also known as JMJD3. a histone-modifying enzyme in the KDM6 subfamily. KDM6B specifically demethylates di- and tri-methylated lysine 27 on histone H3 (H3K27me2/me3), epigenetic marks associated with transcriptional repression. By removing these modifications, KDM6B activates gene expression and plays critical roles in cell differentiation, immune response, aging, and cancer development. It is essential for processes like embryonic development, stem cell pluripotency, and cellular senescence.
The antibody is typically generated by immunizing hosts with recombinant mouse KDM6B protein or peptide epitopes, yielding monoclonal or polyclonal variants. It is validated for applications such as Western blotting, immunoprecipitation, immunofluorescence, and chromatin immunoprecipitation (ChIP). Researchers use it to investigate KDM6B's expression patterns, subcellular localization, and interactions in mouse models. Specificity is confirmed using knockout controls or siRNA-mediated knockdown to ensure minimal cross-reactivity with related proteins like UTX (KDM6A).
This antibody is vital for exploring KDM6B's role in epigenetic regulation, disease mechanisms (e.g., tumorigenesis, neurodevelopmental disorders), and its potential as a therapeutic target. Its utility extends to developmental biology, immunology, and oncology research.
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