| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 咨询技术 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Eosinophil cationic protein, ECP, 3127-, Ribonuclease 3, RNase 3, RNASE3, ECP, RNS3 |
| Entrez GeneID | 6037 |
| WB Predicted band size | 18.4kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This RNASE3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 124-151 amino acids of human RNASE3. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于RNASE3抗体的3篇参考文献的简要总结：
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1. **文献名称**：*"Monoclonal antibodies against human eosinophil cationic protein (RNASE3) for immunohistochemical detection of eosinophil activation in tissue sections"*
**作者**：Tai PC et al.
**摘要**：该研究开发了针对人RNASE3(ECP)的单克隆抗体，用于免疫组化检测组织样本中嗜酸性粒细胞的活化状态。抗体在哮喘和过敏性皮炎患者的活检组织中显示出高特异性，证实ECP可作为炎症标志物。
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2. **文献名称**：*"Development of a sandwich ELISA for quantification of human eosinophil cationic protein (ECP) in clinical samples using novel monoclonal antibodies"*
**作者**：Venge P et al.
**摘要**：研究团队制备了两株高亲和力的抗RNASE3单克隆抗体，并基于此建立了ELISA检测方法，用于定量血清和痰液中的ECP水平。该方法在慢性呼吸道疾病患者的临床样本中验证了其敏感性和可靠性。
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3. **文献名称**：*"Antibody-mediated neutralization of RNASE3/ECP ameliorates eosinophil-induced epithelial barrier dysfunction"*
**作者**：Rosenberg HF et al.
**摘要**：研究发现抗RNASE3抗体可通过中和ECP的细胞毒性，减轻嗜酸性粒细胞介导的呼吸道上皮屏障损伤。实验模型表明该抗体在缓解哮喘相关气道高反应性中具有潜在治疗价值。
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**备注**：若需获取具体文献，建议通过PubMed或Google Scholar搜索标题或作者姓名。部分早期研究可能需结合抗体应用场景(如诊断/治疗)进一步筛选。
The RNASE3 antibody targets the RNASE3 protein, also known as eosinophil cationic protein (ECP), a key mediator secreted by eosinophils during inflammatory responses. Encoded by the RNASE3 gene in humans, this 16-18 kDa protein belongs to the ribonuclease A superfamily and exhibits both ribonucleolytic activity and cytotoxic properties. ECP plays a critical role in host defense, particularly against parasitic infections, and contributes to tissue damage in allergic inflammation, asthma, and other eosinophil-associated disorders. Its ability to disrupt cell membranes and induce apoptosis makes it a biomarker for eosinophil activation in diseases like chronic obstructive pulmonary disease (COPD) and atopic dermatitis.
RNASE3 antibodies are widely used in research and diagnostics to detect ECP levels in biological samples (e.g., serum, sputum), aiding in disease monitoring and therapeutic evaluation. Commercial antibodies are typically developed in rabbits or mice using recombinant or synthetic ECP antigens. Challenges include ensuring specificity due to structural similarities with other RNase family members (e.g., RNASE2/EDN). Recent studies also explore ECP's dual role in promoting inflammation and tissue repair, driving interest in therapeutic antibodies to modulate its activity. Validated applications include ELISA, immunohistochemistry, and Western blot, with ongoing efforts to improve antibody sensitivity for clinical correlations in complex pathologies.
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