| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 技术咨询 | Human,Mouse,Rat |
| FCM | 1/10-1/50 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Histone H2B type 1-J, Histone H2B1, Histone H2Br, H2B/r, HIST1H2BJ, H2BFR |
| Entrez GeneID | 8970 |
| WB Predicted band size | 13.9kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human |
| Immunogen | This HIST1H2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-86 amino acids from the Central region of human HIST1H2BJ. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide. |
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以下是关于HIST1H2BJ抗体的3篇参考文献示例(注：因文献数据库实时更新，以下为假设性示例，建议通过学术引擎进一步验证)：
1. **"Histone H2B variants in chromatin dynamics: Role of HIST1H2BJ in transcriptional regulation"**
*作者：Smith A, et al. (2020)*
**摘要**：本研究探讨了HIST1H2BJ在染色质重塑中的作用，开发了一种特异性多克隆抗体，并通过ChIP-seq和免疫荧光验证其在基因启动子区域的富集，提示其与肿瘤发生相关。
2. **"Development and validation of a monoclonal antibody for detecting HIST1H2BJ in human tissues"**
*作者：Chen L, et al. (2018)*
**摘要**：报道了一种新型抗HIST1H2BJ单克隆抗体的制备，通过蛋白质印迹和免疫组化验证其特异性，应用于肺癌样本中组蛋白表达水平的定量分析。
3. **"Epigenetic dysregulation of HIST1H2BJ in Alzheimer's disease: Insights from antibody-based profiling"**
*作者：Kim Y, et al. (2021)*
**摘要**：利用HIST1H2BJ抗体分析阿尔茨海默病患者脑组织，发现其表达水平与tau蛋白异常磷酸化相关，提示组蛋白变体在神经退行性疾病中的潜在作用。
**提示**：若需实际文献，建议在PubMed或Google Scholar中搜索关键词“HIST1H2BJ antibody”或“H2B antibody”，并筛选涉及抗体开发、表观遗传学或疾病机制的研究。部分研究可能以组蛋白家族整体为对象，需结合全文判断是否包含HIST1H2BJ特异性数据。
The HIST1H2BJ antibody targets a specific histone protein encoded by the HIST1H2BJ gene, which belongs to the histone H2B family. Histones are essential chromatin components that organize DNA into nucleosomes, playing critical roles in gene regulation, DNA repair, and epigenetic modifications. The H2B family, including HIST1H2BJ, contributes to nucleosome structure by forming dimers with H2A histones, stabilizing DNA-histone interactions. HIST1H2BJ is a replication-dependent histone expressed during the S phase of the cell cycle and is involved in maintaining chromatin integrity during DNA replication and transcriptional regulation.
Antibodies against HIST1H2BJ are widely used in research to investigate histone modifications, chromatin dynamics, and epigenetic mechanisms in diseases such as cancer, where aberrant histone expression or post-translational modifications are often observed. These antibodies enable detection of HIST1H2BJ via techniques like Western blotting, immunohistochemistry (IHC), and chromatin immunoprecipitation (ChIP). Specificity is crucial, as H2B variants share high sequence homology; however, well-validated HIST1H2BJ antibodies distinguish this isoform from others. Studies using these antibodies have explored its role in cellular differentiation, apoptosis, and responses to DNA damage. Recent research also links HIST1H2BJ dysregulation to tumor progression, making it a potential biomarker or therapeutic target. Proper validation, including knockout controls or peptide competition assays, ensures reliability in experimental models.
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