| WB | 1/1000 | Human,Mouse,Rat |
| IF | 咨询技术 | Human,Mouse,Rat |
| IHC | 1/100-1/500 | Human,Mouse,Rat |
| ICC | 1/10-1/50 | Human,Mouse,Rat |
| FCM | 咨询技术 | Human,Mouse,Rat |
| Elisa | 咨询技术 | Human,Mouse,Rat |
| Aliases | Transient receptor potential cation channel subfamily M member 8, Long transient receptor potential channel 6, LTrpC-6, LTrpC6, Transient receptor potential p8, Trp-p8, TRPM8, LTRPC6, TRPP8 |
| Entrez GeneID | 79054 |
| WB Predicted band size | 127.7kDa |
| Host/Isotype | Rabbit IgG |
| Antibody Type | Primary antibody |
| Storage | Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles. |
| Species Reactivity | Human, Mouse, Rat |
| Immunogen | This TRPM8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 263-292 amino acids from the Central region of human TRPM8. |
| Formulation | Purified antibody in PBS with 0.05% sodium azide,1%BSA and 50% glycerol.prepared by Saturated Ammonium Sulfate (SAS) . |
+ +
以下是关于TRPM8抗体的3篇参考文献示例，包括文献名称、作者和摘要内容概括：
1. **"A monoclonal antibody targeting TRPM8 modulates cold sensitivity in mice"**
*Authors: Smith A, Jones B, et al.*
**摘要**：研究报道了一种新型单克隆抗体的开发，该抗体特异性靶向TRPM8通道的胞外域。通过在小鼠模型中阻断TRPM8活性，发现该抗体显著降低动物对冷刺激的敏感性，提示其在治疗神经性冷痛中的潜在应用。
2. **"Immunohistochemical analysis of TRPM8 expression in human prostate cancer using a novel antibody"**
*Authors: Lee C, Kim D, et al.*
**摘要**：本文描述了一种高特异性抗TRPM8多克隆抗体的开发，并用于前列腺癌组织的免疫组化分析。结果显示TRPM8在癌变组织中显著高表达，提示其作为生物标志物或治疗靶点的可能性。
3. **"Characterization of TRPM8 channel function using selective blocking antibodies"**
*Authors: Garcia-Sanz N, Valente P, et al.*
**摘要**：研究通过体外实验验证了一种抗TRPM8抗体对通道功能的抑制作用。实验表明，该抗体能有效抑制薄荷醇诱导的钙离子内流，为研究TRPM8在温度感知和疼痛信号传导中的作用提供了工具。
4. **"TRPM8 antibody-based detection of cold-sensing neurons in dorsal root ganglia"**
*Authors: Zhang Y, Wang H, et al.*
**摘要**：利用特异性抗TRPM8抗体标记小鼠背根神经节中的神经元，揭示了冷敏感神经元的分布特征及其与痛觉传导通路的关联，为研究温度感知机制提供了形态学证据。
*注：以上文献信息为示例性质，实际引用时需核对真实出版物。如需具体文献，建议通过PubMed或Web of Science检索关键词“TRPM8 antibody”或联系相关领域数据库。*
TRPM8 (Transient Receptor Potential Melastatin 8) is a calcium-permeable non-selective cation channel belonging to the TRP superfamily. It functions as a primary cold sensor in mammals, activated by temperatures below 26–28°C and cooling agents like menthol or icilin. Expressed predominantly in sensory neurons, prostate, and other tissues, TRPM8 plays roles in thermoregulation, cold pain perception, and physiological processes such as urinary bladder function. Dysregulation of TRPM8 is implicated in pathologies including chronic pain syndromes, prostate cancer, and dry eye disease.
TRPM8 antibodies are essential tools for studying its expression, localization, and function. They enable detection via techniques like Western blotting, immunohistochemistry, and flow cytometry. Developing specific antibodies is challenging due to structural homology among TRP channels and low endogenous TRPM8 levels in some tissues. High-quality antibodies are validated using knockout models or siRNA to confirm target specificity. Applications range from basic research—elucidating TRPM8's role in neuronal signaling or cancer progression—to drug discovery, where they help evaluate therapeutic candidates targeting TRPM8 for conditions like neuropathic pain or overactive bladder. Recent advances also explore TRPM8 antibodies as diagnostic biomarkers for prostate cancer, given its overexpression in malignancies. These reagents continue to drive mechanistic insights and translational opportunities.
×
